Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Serratia proteamaculans Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP424678STJ1
CSB-EP424678STJ1
CSB-BP424678STJ1
CSB-MP424678STJ1
CSB-EP424678STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Chorismate--pyruvate lyase (ubiC) CSB-YP424679STJ
CSB-EP424679STJ
CSB-BP424679STJ
CSB-MP424679STJ
CSB-EP424679STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP424680STJ
CSB-EP424680STJ
CSB-BP424680STJ
CSB-MP424680STJ
CSB-EP424680STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L15 (rplO) CSB-YP424681STJ
CSB-EP424681STJ
CSB-BP424681STJ
CSB-MP424681STJ
CSB-EP424681STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L5 (rplE) CSB-YP424682STJ
CSB-EP424682STJ
CSB-BP424682STJ
CSB-MP424682STJ
CSB-EP424682STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 30S ribosomal protein S3 (rpsC) CSB-YP424683STJ
CSB-EP424683STJ
CSB-BP424683STJ
CSB-MP424683STJ
CSB-EP424683STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L3 (rplC) CSB-YP424684STJ
CSB-EP424684STJ
CSB-BP424684STJ
CSB-MP424684STJ
CSB-EP424684STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0270 protein Spro_4577 (Spro_4577) CSB-YP424685STJ
CSB-EP424685STJ
CSB-BP424685STJ
CSB-MP424685STJ
CSB-EP424685STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 33 kDa chaperonin (hslO) CSB-YP424686STJ
CSB-EP424686STJ
CSB-BP424686STJ
CSB-MP424686STJ
CSB-EP424686STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans HTH-type transcriptional regulator malT (malT), partial CSB-YP424687STJ
CSB-EP424687STJ
CSB-BP424687STJ
CSB-MP424687STJ
CSB-EP424687STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP424688STJ
CSB-EP424688STJ
CSB-BP424688STJ
CSB-MP424688STJ
CSB-EP424688STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Glutamate racemase (murI) CSB-YP424689STJ
CSB-EP424689STJ
CSB-BP424689STJ
CSB-MP424689STJ
CSB-EP424689STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans ATP-dependent protease subunit HslV (hslV) CSB-YP424690STJ
CSB-EP424690STJ
CSB-BP424690STJ
CSB-MP424690STJ
CSB-EP424690STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein-export protein secB (secB) CSB-YP424691STJ
CSB-EP424691STJ
CSB-BP424691STJ
CSB-MP424691STJ
CSB-EP424691STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L28 (rpmB) CSB-YP424692STJ
CSB-EP424692STJ
CSB-BP424692STJ
CSB-MP424692STJ
CSB-EP424692STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribonuclease PH (rph) CSB-YP424693STJ
CSB-EP424693STJ
CSB-BP424693STJ
CSB-MP424693STJ
CSB-EP424693STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable GTP-binding protein EngB (engB) CSB-YP424694STJ
CSB-EP424694STJ
CSB-BP424694STJ
CSB-MP424694STJ
CSB-EP424694STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP424695RIL
CSB-EP424695RIL
CSB-BP424695RIL
CSB-MP424695RIL
CSB-EP424695RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari 30S ribosomal protein S18 (rpsR) CSB-YP424696RIL
CSB-EP424696RIL
CSB-BP424696RIL
CSB-MP424696RIL
CSB-EP424696RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP424697RIL
CSB-EP424697RIL
CSB-BP424697RIL
CSB-MP424697RIL
CSB-EP424697RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>