Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella sediminis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP424538STR
CSB-EP424538STR
CSB-BP424538STR
CSB-MP424538STR
CSB-EP424538STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Probable intracellular septation protein A (Ssed_1670), partial CSB-YP424539STR1
CSB-EP424539STR1
CSB-BP424539STR1
CSB-MP424539STR1
CSB-EP424539STR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis UPF0352 protein Ssed_1809 (Ssed_1809) CSB-YP424540STR
CSB-EP424540STR
CSB-BP424540STR
CSB-MP424540STR
CSB-EP424540STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP424541STR
CSB-EP424541STR
CSB-BP424541STR
CSB-MP424541STR
CSB-EP424541STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Phosphomethylpyrimidine synthase (thiC), partial CSB-YP424542STR
CSB-EP424542STR
CSB-BP424542STR
CSB-MP424542STR
CSB-EP424542STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Electron transport complex protein RnfA (rnfA), partial CSB-YP424543STR1
CSB-EP424543STR1
CSB-BP424543STR1
CSB-MP424543STR1
CSB-EP424543STR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Protein CrcB homolog (crcB), partial CSB-YP424544STR1
CSB-EP424544STR1
CSB-BP424544STR1
CSB-MP424544STR1
CSB-EP424544STR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Cytidylate kinase (cmk) CSB-YP424545STR
CSB-EP424545STR
CSB-BP424545STR
CSB-MP424545STR
CSB-EP424545STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP424546STR
CSB-EP424546STR
CSB-BP424546STR
CSB-MP424546STR
CSB-EP424546STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP424547STR
CSB-EP424547STR
CSB-BP424547STR
CSB-MP424547STR
CSB-EP424547STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis UPF0597 protein Ssed_2537 (Ssed_2537) CSB-YP424548STR
CSB-EP424548STR
CSB-BP424548STR
CSB-MP424548STR
CSB-EP424548STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP424549STR
CSB-EP424549STR
CSB-BP424549STR
CSB-MP424549STR
CSB-EP424549STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Biotin synthase (bioB) CSB-YP424550STR
CSB-EP424550STR
CSB-BP424550STR
CSB-MP424550STR
CSB-EP424550STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP424551STR
CSB-EP424551STR
CSB-BP424551STR
CSB-MP424551STR
CSB-EP424551STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Recombination protein RecR (recR) CSB-YP424552STR
CSB-EP424552STR
CSB-BP424552STR
CSB-MP424552STR
CSB-EP424552STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Chaperone protein hscA homolog (hscA), partial CSB-YP424553STR
CSB-EP424553STR
CSB-BP424553STR
CSB-MP424553STR
CSB-EP424553STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Probable phosphatase Ssed_2939 (Ssed_2939) CSB-YP424554STR
CSB-EP424554STR
CSB-BP424554STR
CSB-MP424554STR
CSB-EP424554STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Protein Xni (xni) CSB-YP424555STR
CSB-EP424555STR
CSB-BP424555STR
CSB-MP424555STR
CSB-EP424555STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Chaperone protein DnaJ (dnaJ) CSB-YP424556STR
CSB-EP424556STR
CSB-BP424556STR
CSB-MP424556STR
CSB-EP424556STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Glutamate 5-kinase (proB) CSB-YP424557STR
CSB-EP424557STR
CSB-BP424557STR
CSB-MP424557STR
CSB-EP424557STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>