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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 Probable cytosol aminopeptidase (pepA) CSB-YP425140EJD
CSB-EP425140EJD
CSB-BP425140EJD
CSB-MP425140EJD
CSB-EP425140EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP425141EJD
CSB-EP425141EJD
CSB-BP425141EJD
CSB-MP425141EJD
CSB-EP425141EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 30S ribosomal protein S20 (rpsT) CSB-YP425142EJF
CSB-EP425142EJF
CSB-BP425142EJF
CSB-MP425142EJF
CSB-EP425142EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Crotonobetainyl-CoA:carnitine CoA-transferase (caiB) CSB-YP425143EJF
CSB-EP425143EJF
CSB-BP425143EJF
CSB-MP425143EJF
CSB-EP425143EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein ApaG (apaG) CSB-YP425144EJF
CSB-EP425144EJF
CSB-BP425144EJF
CSB-MP425144EJF
CSB-EP425144EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 2-isopropylmalate synthase (leuA) CSB-YP425145EJF
CSB-EP425145EJF
CSB-BP425145EJF
CSB-MP425145EJF
CSB-EP425145EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein translocase subunit SecA (secA), partial CSB-YP425146EJF
CSB-EP425146EJF
CSB-BP425146EJF
CSB-MP425146EJF
CSB-EP425146EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP425147EJF
CSB-EP425147EJF
CSB-BP425147EJF
CSB-MP425147EJF
CSB-EP425147EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0325 protein yaeH (yaeH) CSB-YP425148EJF
CSB-EP425148EJF
CSB-BP425148EJF
CSB-MP425148EJF
CSB-EP425148EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Outer membrane protein assembly factor yaeT (yaeT) CSB-YP425149EJF
CSB-EP425149EJF
CSB-BP425149EJF
CSB-MP425149EJF
CSB-EP425149EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ribonuclease H (rnhA) CSB-YP425150EJF
CSB-EP425150EJF
CSB-BP425150EJF
CSB-MP425150EJF
CSB-EP425150EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Probable HTH-type transcriptional regulator matA homolog (matA) CSB-YP425151EJF
CSB-EP425151EJF
CSB-BP425151EJF
CSB-MP425151EJF
CSB-EP425151EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase CSB-YP425152EJF
CSB-EP425152EJF
CSB-BP425152EJF
CSB-MP425152EJF
CSB-EP425152EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Transcriptional repressor frmR (frmR) CSB-YP425153EJF
CSB-EP425153EJF
CSB-BP425153EJF
CSB-MP425153EJF
CSB-EP425153EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 tRNA 2-selenouridine synthase (selU) CSB-YP425154EJF
CSB-EP425154EJF
CSB-BP425154EJF
CSB-MP425154EJF
CSB-EP425154EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Carboxylate-amine ligase YbdK (ybdK) CSB-YP425155EJF
CSB-EP425155EJF
CSB-BP425155EJF
CSB-MP425155EJF
CSB-EP425155EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glutamine--tRNA ligase (glnS) CSB-YP425156EJF
CSB-EP425156EJF
CSB-BP425156EJF
CSB-MP425156EJF
CSB-EP425156EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP425157EJF
CSB-EP425157EJF
CSB-BP425157EJF
CSB-MP425157EJF
CSB-EP425157EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0194 membrane protein ybhG (ybhG) CSB-YP425158EJF
CSB-EP425158EJF
CSB-BP425158EJF
CSB-MP425158EJF
CSB-EP425158EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP425159EJF
CSB-EP425159EJF
CSB-BP425159EJF
CSB-MP425159EJF
CSB-EP425159EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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