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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arcobacter butzleri Cytochrome c-552 (nrfA) CSB-YP424337AUY
CSB-EP424337AUY
CSB-BP424337AUY
CSB-MP424337AUY
CSB-EP424337AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Leucine--tRNA ligase (leuS), partial CSB-YP424338AUY
CSB-EP424338AUY
CSB-BP424338AUY
CSB-MP424338AUY
CSB-EP424338AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 2-isopropylmalate synthase (leuA), partial CSB-YP424339AUY
CSB-EP424339AUY
CSB-BP424339AUY
CSB-MP424339AUY
CSB-EP424339AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 30S ribosomal protein S2 (rpsB) CSB-YP424340AUY
CSB-EP424340AUY
CSB-BP424340AUY
CSB-MP424340AUY
CSB-EP424340AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Bifunctional protein FolD (folD) CSB-YP424341AUY
CSB-EP424341AUY
CSB-BP424341AUY
CSB-MP424341AUY
CSB-EP424341AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 50S ribosomal protein L2 (rplB) CSB-YP424342AUY
CSB-EP424342AUY
CSB-BP424342AUY
CSB-MP424342AUY
CSB-EP424342AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 30S ribosomal protein S14 type Z (rpsZ) CSB-YP424343AUY
CSB-EP424343AUY
CSB-BP424343AUY
CSB-MP424343AUY
CSB-EP424343AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Alanine racemase (alr) CSB-YP424344AUY
CSB-EP424344AUY
CSB-BP424344AUY
CSB-MP424344AUY
CSB-EP424344AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Urease accessory protein UreG (ureG) CSB-YP424345AUY
CSB-EP424345AUY
CSB-BP424345AUY
CSB-MP424345AUY
CSB-EP424345AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri ATP-dependent protease subunit HslV (hslV) CSB-YP424346AUY
CSB-EP424346AUY
CSB-BP424346AUY
CSB-MP424346AUY
CSB-EP424346AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP424347AUY
CSB-EP424347AUY
CSB-BP424347AUY
CSB-MP424347AUY
CSB-EP424347AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP424348AUY
CSB-EP424348AUY
CSB-BP424348AUY
CSB-MP424348AUY
CSB-EP424348AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP424349AUY
CSB-EP424349AUY
CSB-BP424349AUY
CSB-MP424349AUY
CSB-EP424349AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Catalase-peroxidase (katG), partial CSB-YP424350AUY
CSB-EP424350AUY
CSB-BP424350AUY
CSB-MP424350AUY
CSB-EP424350AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 50S ribosomal protein L27 (rpmA) CSB-YP424351AUY
CSB-EP424351AUY
CSB-BP424351AUY
CSB-MP424351AUY
CSB-EP424351AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Protease HtpX homolog (htpX), partial CSB-YP424352AUY1
CSB-EP424352AUY1
CSB-BP424352AUY1
CSB-MP424352AUY1
CSB-EP424352AUY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri ATP synthase subunit c (atpE), partial CSB-YP424353AUY1
CSB-EP424353AUY1
CSB-BP424353AUY1
CSB-MP424353AUY1
CSB-EP424353AUY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Bifunctional protein HldE (hldE) CSB-YP424354AUY
CSB-EP424354AUY
CSB-BP424354AUY
CSB-MP424354AUY
CSB-EP424354AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP424355AUY
CSB-EP424355AUY
CSB-BP424355AUY
CSB-MP424355AUY
CSB-EP424355AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 50S ribosomal protein L1 (rplA) CSB-YP424356AUY
CSB-EP424356AUY
CSB-BP424356AUY
CSB-MP424356AUY
CSB-EP424356AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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