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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arcobacter butzleri 30S ribosomal protein S12 (rpsL) CSB-YP424357AUY
CSB-EP424357AUY
CSB-BP424357AUY
CSB-MP424357AUY
CSB-EP424357AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Dihydroxy-acid dehydratase (ilvD), partial CSB-YP424358AUY
CSB-EP424358AUY
CSB-BP424358AUY
CSB-MP424358AUY
CSB-EP424358AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP424359AUY
CSB-EP424359AUY
CSB-BP424359AUY
CSB-MP424359AUY
CSB-EP424359AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP424360AUY
CSB-EP424360AUY
CSB-BP424360AUY
CSB-MP424360AUY
CSB-EP424360AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP424361AUY
CSB-EP424361AUY
CSB-BP424361AUY
CSB-MP424361AUY
CSB-EP424361AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP424362RMR
CSB-EP424362RMR
CSB-BP424362RMR
CSB-MP424362RMR
CSB-EP424362RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S6 (rpsF) CSB-YP424363RMR
CSB-EP424363RMR
CSB-BP424363RMR
CSB-MP424363RMR
CSB-EP424363RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP424364RMR
CSB-EP424364RMR
CSB-BP424364RMR
CSB-MP424364RMR
CSB-EP424364RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L10 (rplJ) CSB-YP424365RMR
CSB-EP424365RMR
CSB-BP424365RMR
CSB-MP424365RMR
CSB-EP424365RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP424366RMR
CSB-EP424366RMR
CSB-BP424366RMR
CSB-MP424366RMR
CSB-EP424366RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Co-chaperone protein hscB homolog (hscB) CSB-YP424367RMR
CSB-EP424367RMR
CSB-BP424367RMR
CSB-MP424367RMR
CSB-EP424367RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Putative pterin-4-alpha-carbinolamine dehydratase (A1E_01360) CSB-YP424368RMR
CSB-EP424368RMR
CSB-BP424368RMR
CSB-MP424368RMR
CSB-EP424368RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis D-alanine--D-alanine ligase (ddl) CSB-YP424369RMR
CSB-EP424369RMR
CSB-BP424369RMR
CSB-MP424369RMR
CSB-EP424369RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Ribonuclease P protein component (rnpA) CSB-YP424370RMR
CSB-EP424370RMR
CSB-BP424370RMR
CSB-MP424370RMR
CSB-EP424370RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP424371RMR
CSB-EP424371RMR
CSB-BP424371RMR
CSB-MP424371RMR
CSB-EP424371RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis UvrABC system protein C (uvrC), partial CSB-YP424372RMR
CSB-EP424372RMR
CSB-BP424372RMR
CSB-MP424372RMR
CSB-EP424372RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L36 (rpmJ) CSB-YP424373RMR
CSB-EP424373RMR
CSB-BP424373RMR
CSB-MP424373RMR
CSB-EP424373RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis SsrA-binding protein (smpB) CSB-YP424374RMR
CSB-EP424374RMR
CSB-BP424374RMR
CSB-MP424374RMR
CSB-EP424374RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Lipoprotein signal peptidase (lspA), partial CSB-YP424375RMR1
CSB-EP424375RMR1
CSB-BP424375RMR1
CSB-MP424375RMR1
CSB-EP424375RMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Putative membrane protein insertion efficiency factor (A1E_03705) CSB-YP424376RMR
CSB-EP424376RMR
CSB-BP424376RMR
CSB-MP424376RMR
CSB-EP424376RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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