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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Azorhizobium caulinodans Bifunctional enzyme IspD/IspF (ispDF) CSB-YP423837DPR
CSB-EP423837DPR
CSB-BP423837DPR
CSB-MP423837DPR
CSB-EP423837DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Ribosome biogenesis protein BOP1 homolog (CHLREDRAFT_111274), partial CSB-YP423838DSJ
CSB-EP423838DSJ
CSB-BP423838DSJ
CSB-MP423838DSJ
CSB-EP423838DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Elongation factor Ts, mitochondrial (EFTS), partial CSB-YP423839DSJ
CSB-EP423839DSJ
CSB-BP423839DSJ
CSB-MP423839DSJ
CSB-EP423839DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Protein translocase subunit SecA (secA), partial CSB-YP423840DPR
CSB-EP423840DPR
CSB-BP423840DPR
CSB-MP423840DPR
CSB-EP423840DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Biotin synthase (bioB) CSB-YP423841DPR
CSB-EP423841DPR
CSB-BP423841DPR
CSB-MP423841DPR
CSB-EP423841DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans UPF0178 protein AZC_4000 (AZC_4000) CSB-YP423842DPR
CSB-EP423842DPR
CSB-BP423842DPR
CSB-MP423842DPR
CSB-EP423842DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP423843DPR
CSB-EP423843DPR
CSB-BP423843DPR
CSB-MP423843DPR
CSB-EP423843DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP423844DPR
CSB-EP423844DPR
CSB-BP423844DPR
CSB-MP423844DPR
CSB-EP423844DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans PKHD-type hydroxylase AZC_3753 (AZC_3753) CSB-YP423845DPR
CSB-EP423845DPR
CSB-BP423845DPR
CSB-MP423845DPR
CSB-EP423845DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Chaperone protein DnaK (dnaK), partial CSB-YP423846DPR
CSB-EP423846DPR
CSB-BP423846DPR
CSB-MP423846DPR
CSB-EP423846DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP423847DPR
CSB-EP423847DPR
CSB-BP423847DPR
CSB-MP423847DPR
CSB-EP423847DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Primary ciliary dyskinesia protein 1 homolog (FAP221), partial CSB-YP423848DSJ
CSB-EP423848DSJ
CSB-BP423848DSJ
CSB-MP423848DSJ
CSB-EP423848DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Lipoyl synthase, mitochondrial (LIP1), partial CSB-YP423849DSJ
CSB-EP423849DSJ
CSB-BP423849DSJ
CSB-MP423849DSJ
CSB-EP423849DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frankia sp. UvrABC system protein C (uvrC), partial CSB-YP423850FDY
CSB-EP423850FDY
CSB-BP423850FDY
CSB-MP423850FDY
CSB-EP423850FDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frankia sp. Probable rRNA maturation factor (Franean1_2113) CSB-YP423851FDY
CSB-EP423851FDY
CSB-BP423851FDY
CSB-MP423851FDY
CSB-EP423851FDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frankia sp. Probable transcriptional regulatory protein Franean1_5147 (Franean1_5147) CSB-YP423852FDY
CSB-EP423852FDY
CSB-BP423852FDY
CSB-MP423852FDY
CSB-EP423852FDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frankia sp. 50S ribosomal protein L27 (rpmA) CSB-YP423853FDY
CSB-EP423853FDY
CSB-BP423853FDY
CSB-MP423853FDY
CSB-EP423853FDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frankia sp. UPF0753 protein Franean1_5483 (Franean1_5483), partial CSB-YP423854FDY
CSB-EP423854FDY
CSB-BP423854FDY
CSB-MP423854FDY
CSB-EP423854FDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frankia sp. 3-isopropylmalate dehydrogenase (leuB) CSB-YP423855FDY
CSB-EP423855FDY
CSB-BP423855FDY
CSB-MP423855FDY
CSB-EP423855FDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frankia sp. Ribosome maturation factor RimM (rimM) CSB-YP423856FDY
CSB-EP423856FDY
CSB-BP423856FDY
CSB-MP423856FDY
CSB-EP423856FDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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