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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ignicoccus hospitalis Riboflavin kinase (ribK) CSB-YP426240ILH
CSB-EP426240ILH
CSB-BP426240ILH
CSB-MP426240ILH
CSB-EP426240ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Ribonuclease P protein component 1 (rnp1) CSB-YP426241ILH
CSB-EP426241ILH
CSB-BP426241ILH
CSB-MP426241ILH
CSB-EP426241ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP426242ILH
CSB-EP426242ILH
CSB-BP426242ILH
CSB-MP426242ILH
CSB-EP426242ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Cytidylate kinase (cmk) CSB-YP426243ILH
CSB-EP426243ILH
CSB-BP426243ILH
CSB-MP426243ILH
CSB-EP426243ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis 30S ribosomal protein S15P/S13e (rps15p) CSB-YP426244ILH
CSB-EP426244ILH
CSB-BP426244ILH
CSB-MP426244ILH
CSB-EP426244ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP426245ILH
CSB-EP426245ILH
CSB-BP426245ILH
CSB-MP426245ILH
CSB-EP426245ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis DNA repair and recombination protein radA (radA) CSB-YP426246ILH
CSB-EP426246ILH
CSB-BP426246ILH
CSB-MP426246ILH
CSB-EP426246ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Translation initiation factor 5A (eIF5A) CSB-YP426247ILH
CSB-EP426247ILH
CSB-BP426247ILH
CSB-MP426247ILH
CSB-EP426247ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis UPF0173 metal-dependent hydrolase Igni_1254 (Igni_1254) CSB-YP426248ILH
CSB-EP426248ILH
CSB-BP426248ILH
CSB-MP426248ILH
CSB-EP426248ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP426249ILH
CSB-EP426249ILH
CSB-BP426249ILH
CSB-MP426249ILH
CSB-EP426249ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP426250ILH
CSB-EP426250ILH
CSB-BP426250ILH
CSB-MP426250ILH
CSB-EP426250ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Membrane protein insertase YidC (yidC), partial CSB-YP426251CWS1
CSB-EP426251CWS1
CSB-BP426251CWS1
CSB-MP426251CWS1
CSB-EP426251CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Low affinity potassium transport system protein kup (kup), partial CSB-YP426252CWS
CSB-EP426252CWS
CSB-BP426252CWS
CSB-MP426252CWS
CSB-EP426252CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP426253CWS
CSB-EP426253CWS
CSB-BP426253CWS
CSB-MP426253CWS
CSB-EP426253CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP426254CWS
CSB-EP426254CWS
CSB-BP426254CWS
CSB-MP426254CWS
CSB-EP426254CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP426255CWS
CSB-EP426255CWS
CSB-BP426255CWS
CSB-MP426255CWS
CSB-EP426255CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Uracil phosphoribosyltransferase (upp) CSB-YP426256CWS
CSB-EP426256CWS
CSB-BP426256CWS
CSB-MP426256CWS
CSB-EP426256CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP426257CWS
CSB-EP426257CWS
CSB-BP426257CWS
CSB-MP426257CWS
CSB-EP426257CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP426258CWS
CSB-EP426258CWS
CSB-BP426258CWS
CSB-MP426258CWS
CSB-EP426258CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP426259CWS
CSB-EP426259CWS
CSB-BP426259CWS
CSB-MP426259CWS
CSB-EP426259CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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