Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella pealeana 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP426839STP
CSB-EP426839STP
CSB-BP426839STP
CSB-MP426839STP
CSB-EP426839STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP426840STP
CSB-EP426840STP
CSB-BP426840STP
CSB-MP426840STP
CSB-EP426840STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Putative pterin-4-alpha-carbinolamine dehydratase (Spea_1449) CSB-YP426841STP
CSB-EP426841STP
CSB-BP426841STP
CSB-MP426841STP
CSB-EP426841STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Co-chaperone protein hscB homolog (hscB) CSB-YP426842STP
CSB-EP426842STP
CSB-BP426842STP
CSB-MP426842STP
CSB-EP426842STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Tryptophan synthase beta chain (trpB) CSB-YP426843STP
CSB-EP426843STP
CSB-BP426843STP
CSB-MP426843STP
CSB-EP426843STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Cell division protein ZipA homolog (zipA), partial CSB-YP426844STP
CSB-EP426844STP
CSB-BP426844STP
CSB-MP426844STP
CSB-EP426844STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Biotin synthase (bioB) CSB-YP426845STP
CSB-EP426845STP
CSB-BP426845STP
CSB-MP426845STP
CSB-EP426845STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana UvrABC system protein C (uvrC), partial CSB-YP426846STP
CSB-EP426846STP
CSB-BP426846STP
CSB-MP426846STP
CSB-EP426846STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP426847STP
CSB-EP426847STP
CSB-BP426847STP
CSB-MP426847STP
CSB-EP426847STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 7-cyano-7-deazaguanine synthase (queC) CSB-YP426848STP
CSB-EP426848STP
CSB-BP426848STP
CSB-MP426848STP
CSB-EP426848STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP426849STP
CSB-EP426849STP
CSB-BP426849STP
CSB-MP426849STP
CSB-EP426849STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Phosphomethylpyrimidine synthase (thiC), partial CSB-YP426850STP
CSB-EP426850STP
CSB-BP426850STP
CSB-MP426850STP
CSB-EP426850STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Cell division topological specificity factor (minE) CSB-YP426851STP
CSB-EP426851STP
CSB-BP426851STP
CSB-MP426851STP
CSB-EP426851STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Biosynthetic arginine decarboxylase (speA), partial CSB-YP426852STP
CSB-EP426852STP
CSB-BP426852STP
CSB-MP426852STP
CSB-EP426852STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana UPF0176 protein Spea_2524 (Spea_2524) CSB-YP426853STP
CSB-EP426853STP
CSB-BP426853STP
CSB-MP426853STP
CSB-EP426853STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP426854STP
CSB-EP426854STP
CSB-BP426854STP
CSB-MP426854STP
CSB-EP426854STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP426855STP
CSB-EP426855STP
CSB-BP426855STP
CSB-MP426855STP
CSB-EP426855STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Bifunctional protein FolD (folD) CSB-YP426856STP
CSB-EP426856STP
CSB-BP426856STP
CSB-MP426856STP
CSB-EP426856STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Ribonuclease HII (rnhB) CSB-YP426857STP
CSB-EP426857STP
CSB-BP426857STP
CSB-MP426857STP
CSB-EP426857STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Protein syd (syd) CSB-YP426858STP
CSB-EP426858STP
CSB-BP426858STP
CSB-MP426858STP
CSB-EP426858STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>