Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP426099EJD
CSB-EP426099EJD
CSB-BP426099EJD
CSB-MP426099EJD
CSB-EP426099EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Alanine--tRNA ligase (alaS), partial CSB-YP426100EJD
CSB-EP426100EJD
CSB-BP426100EJD
CSB-MP426100EJD
CSB-EP426100EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 5'/3'-nucleotidase SurE (surE) CSB-YP426101EJD
CSB-EP426101EJD
CSB-BP426101EJD
CSB-MP426101EJD
CSB-EP426101EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP426102EJD
CSB-EP426102EJD
CSB-BP426102EJD
CSB-MP426102EJD
CSB-EP426102EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 L-fucose isomerase (fucI) CSB-YP426103EJD
CSB-EP426103EJD
CSB-BP426103EJD
CSB-MP426103EJD
CSB-EP426103EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Bifunctional protein aas (aas), partial CSB-YP426104EJD1
CSB-EP426104EJD1
CSB-BP426104EJD1
CSB-MP426104EJD1
CSB-EP426104EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0114 protein YqhA (yqhA), partial CSB-YP426105EJD1
CSB-EP426105EJD1
CSB-BP426105EJD1
CSB-MP426105EJD1
CSB-EP426105EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP426106EJD1
CSB-EP426106EJD1
CSB-BP426106EJD1
CSB-MP426106EJD1
CSB-EP426106EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Threonine/serine transporter TdcC (tdcC), partial CSB-YP426107EJD
CSB-EP426107EJD
CSB-BP426107EJD
CSB-MP426107EJD
CSB-EP426107EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Polyribonucleotide nucleotidyltransferase (pnp) CSB-YP426108EJD
CSB-EP426108EJD
CSB-BP426108EJD
CSB-MP426108EJD
CSB-EP426108EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 GTPase obg (obg) CSB-YP426109EJD
CSB-EP426109EJD
CSB-BP426109EJD
CSB-MP426109EJD
CSB-EP426109EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Putative sialic acid transporter (nanT), partial CSB-YP426110EJD
CSB-EP426110EJD
CSB-BP426110EJD
CSB-MP426110EJD
CSB-EP426110EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 DNA-binding protein fis (fis) CSB-YP426111EJD
CSB-EP426111EJD
CSB-BP426111EJD
CSB-MP426111EJD
CSB-EP426111EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP426112EJD
CSB-EP426112EJD
CSB-BP426112EJD
CSB-MP426112EJD
CSB-EP426112EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L6 (rplF) CSB-YP426113EJD
CSB-EP426113EJD
CSB-BP426113EJD
CSB-MP426113EJD
CSB-EP426113EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L16 (rplP) CSB-YP426114EJD
CSB-EP426114EJD
CSB-BP426114EJD
CSB-MP426114EJD
CSB-EP426114EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Elongation factor Tu 1 (tuf1) CSB-YP426115EJD
CSB-EP426115EJD
CSB-BP426115EJD
CSB-MP426115EJD
CSB-EP426115EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glutathione-regulated potassium-efflux system protein KefB (kefB), partial CSB-YP426116EJD
CSB-EP426116EJD
CSB-BP426116EJD
CSB-MP426116EJD
CSB-EP426116EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ferrous iron transport protein C (feoC) CSB-YP426117EJD
CSB-EP426117EJD
CSB-BP426117EJD
CSB-MP426117EJD
CSB-EP426117EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP426118EJD
CSB-EP426118EJD
CSB-BP426118EJD
CSB-MP426118EJD
CSB-EP426118EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>