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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 Protease HtpX (htpX), partial CSB-YP426179EJF1
CSB-EP426179EJF1
CSB-BP426179EJF1
CSB-MP426179EJF1
CSB-EP426179EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Flagellar transcriptional regulator FlhD (flhD) CSB-YP426180EJF
CSB-EP426180EJF
CSB-BP426180EJF
CSB-MP426180EJF
CSB-EP426180EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Flagellar hook-basal body complex protein FliE (fliE) CSB-YP426181EJF
CSB-EP426181EJF
CSB-BP426181EJF
CSB-MP426181EJF
CSB-EP426181EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0265 protein yeeX (yeeX) CSB-YP426182EJF
CSB-EP426182EJF
CSB-BP426182EJF
CSB-MP426182EJF
CSB-EP426182EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Multidrug resistance protein MdtC (mdtC), partial CSB-YP426183EJF
CSB-EP426183EJF
CSB-BP426183EJF
CSB-MP426183EJF
CSB-EP426183EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Hydroxyethylthiazole kinase (thiM) CSB-YP426184EJF
CSB-EP426184EJF
CSB-BP426184EJF
CSB-MP426184EJF
CSB-EP426184EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0299 membrane protein yohJ (yohJ), partial CSB-YP426185EJF1
CSB-EP426185EJF1
CSB-BP426185EJF1
CSB-MP426185EJF1
CSB-EP426185EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Elongation factor P-like protein (yeiP) CSB-YP426186EJF
CSB-EP426186EJF
CSB-BP426186EJF
CSB-MP426186EJF
CSB-EP426186EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Nucleoside triphosphatase nudI (nudI) CSB-YP426187EJF
CSB-EP426187EJF
CSB-BP426187EJF
CSB-MP426187EJF
CSB-EP426187EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 o-succinylbenzoate synthase (menC) CSB-YP426188EJF
CSB-EP426188EJF
CSB-BP426188EJF
CSB-MP426188EJF
CSB-EP426188EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP426189EJF
CSB-EP426189EJF
CSB-BP426189EJF
CSB-MP426189EJF
CSB-EP426189EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 D-serine dehydratase (dsdA) CSB-YP426190EJF
CSB-EP426190EJF
CSB-BP426190EJF
CSB-MP426190EJF
CSB-EP426190EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein CysZ (cysZ), partial CSB-YP426191EJF1
CSB-EP426191EJF1
CSB-BP426191EJF1
CSB-MP426191EJF1
CSB-EP426191EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 GDP-mannose pyrophosphatase nudK (nudK) CSB-YP426192EJF
CSB-EP426192EJF
CSB-BP426192EJF
CSB-MP426192EJF
CSB-EP426192EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Chaperone protein hscA (hscA) CSB-YP426193EJF
CSB-EP426193EJF
CSB-BP426193EJF
CSB-MP426193EJF
CSB-EP426193EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase (hcaB) CSB-YP426194EJF
CSB-EP426194EJF
CSB-BP426194EJF
CSB-MP426194EJF
CSB-EP426194EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ribosome maturation factor RimM (rimM) CSB-YP426195EJF
CSB-EP426195EJF
CSB-BP426195EJF
CSB-MP426195EJF
CSB-EP426195EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 S-ribosylhomocysteine lyase (luxS) CSB-YP426196EJF
CSB-EP426196EJF
CSB-BP426196EJF
CSB-MP426196EJF
CSB-EP426196EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP426197EJF
CSB-EP426197EJF
CSB-BP426197EJF
CSB-MP426197EJF
CSB-EP426197EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Cell division protein FtsB (ftsB), partial CSB-YP426198EJF
CSB-EP426198EJF
CSB-BP426198EJF
CSB-MP426198EJF
CSB-EP426198EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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