Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 Transcriptional repressor frmR (frmR) CSB-YP426059EJD
CSB-EP426059EJD
CSB-BP426059EJD
CSB-MP426059EJD
CSB-EP426059EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 tRNA sulfurtransferase (thiI) CSB-YP426060EJD
CSB-EP426060EJD
CSB-BP426060EJD
CSB-MP426060EJD
CSB-EP426060EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ureidoglycolate hydrolase (allA) CSB-YP426061EJD
CSB-EP426061EJD
CSB-BP426061EJD
CSB-MP426061EJD
CSB-EP426061EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CSB-YP426062EJD
CSB-EP426062EJD
CSB-BP426062EJD
CSB-MP426062EJD
CSB-EP426062EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 LPS-assembly lipoprotein lptE (lptE) CSB-YP426063EJD
CSB-EP426063EJD
CSB-BP426063EJD
CSB-MP426063EJD
CSB-EP426063EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Potassium-transporting ATPase B chain (kdpB), partial CSB-YP426064EJD1
CSB-EP426064EJD1
CSB-BP426064EJD1
CSB-MP426064EJD1
CSB-EP426064EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 PKHD-type hydroxylase ybiX (ybiX) CSB-YP426065EJD
CSB-EP426065EJD
CSB-BP426065EJD
CSB-MP426065EJD
CSB-EP426065EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP426066EJD
CSB-EP426066EJD
CSB-BP426066EJD
CSB-MP426066EJD
CSB-EP426066EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Uncharacterized MFS-type transporter ycaD (ycaD), partial CSB-YP426067EJD
CSB-EP426067EJD
CSB-BP426067EJD
CSB-MP426067EJD
CSB-EP426067EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP426068EJD
CSB-EP426068EJD
CSB-BP426068EJD
CSB-MP426068EJD
CSB-EP426068EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Macrodomain Ter protein (matP) CSB-YP426069EJD
CSB-EP426069EJD
CSB-BP426069EJD
CSB-MP426069EJD
CSB-EP426069EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Flavoprotein wrbA (wrbA) CSB-YP426070EJD
CSB-EP426070EJD
CSB-BP426070EJD
CSB-MP426070EJD
CSB-EP426070EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Multidrug resistance protein MdtG (mdtG), partial CSB-YP426071EJD
CSB-EP426071EJD
CSB-BP426071EJD
CSB-MP426071EJD
CSB-EP426071EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP426072EJD
CSB-EP426072EJD
CSB-BP426072EJD
CSB-MP426072EJD
CSB-EP426072EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 High frequency lysogenization protein HflD (hflD) CSB-YP426073EJD
CSB-EP426073EJD
CSB-BP426073EJD
CSB-MP426073EJD
CSB-EP426073EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Na (+)/H (+) antiporter nhaB, partial CSB-YP426074EJD
CSB-EP426074EJD
CSB-BP426074EJD
CSB-MP426074EJD
CSB-EP426074EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Probable intracellular septation protein A (yciB), partial CSB-YP426075EJD1
CSB-EP426075EJD1
CSB-BP426075EJD1
CSB-MP426075EJD1
CSB-EP426075EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Gamma-aminobutyraldehyde dehydrogenase (prr) CSB-YP426076EJD
CSB-EP426076EJD
CSB-BP426076EJD
CSB-MP426076EJD
CSB-EP426076EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Uncharacterized aldolase lsrF (lsrF) CSB-YP426077EJD
CSB-EP426077EJD
CSB-BP426077EJD
CSB-MP426077EJD
CSB-EP426077EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Electron transport complex protein RnfE (rnfE), partial CSB-YP426078EJD1
CSB-EP426078EJD1
CSB-BP426078EJD1
CSB-MP426078EJD1
CSB-EP426078EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>