Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfococcus oleovorans 30S ribosomal protein S9 (rpsI) CSB-YP427840DHW
CSB-EP427840DHW
CSB-BP427840DHW
CSB-MP427840DHW
CSB-EP427840DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP427841DHW
CSB-EP427841DHW
CSB-BP427841DHW
CSB-MP427841DHW
CSB-EP427841DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP427842DHW
CSB-EP427842DHW
CSB-BP427842DHW
CSB-MP427842DHW
CSB-EP427842DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Lon protease (lon), partial CSB-YP427843DHW
CSB-EP427843DHW
CSB-BP427843DHW
CSB-MP427843DHW
CSB-EP427843DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP427844DHW
CSB-EP427844DHW
CSB-BP427844DHW
CSB-MP427844DHW
CSB-EP427844DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans DNA mismatch repair protein MutS (mutS), partial CSB-YP427845DHW
CSB-EP427845DHW
CSB-BP427845DHW
CSB-MP427845DHW
CSB-EP427845DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP427846DHW
CSB-EP427846DHW
CSB-BP427846DHW
CSB-MP427846DHW
CSB-EP427846DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP427847DHW
CSB-EP427847DHW
CSB-BP427847DHW
CSB-MP427847DHW
CSB-EP427847DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Hydroxylamine reductase (hcp), partial CSB-YP427848DHW
CSB-EP427848DHW
CSB-BP427848DHW
CSB-MP427848DHW
CSB-EP427848DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Ribosome-binding factor A (rbfA) CSB-YP427849DHW
CSB-EP427849DHW
CSB-BP427849DHW
CSB-MP427849DHW
CSB-EP427849DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP427850DHW
CSB-EP427850DHW
CSB-BP427850DHW
CSB-MP427850DHW
CSB-EP427850DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP427851DHW
CSB-EP427851DHW
CSB-BP427851DHW
CSB-MP427851DHW
CSB-EP427851DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP427852DHW
CSB-EP427852DHW
CSB-BP427852DHW
CSB-MP427852DHW
CSB-EP427852DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Arginine--tRNA ligase (argS), partial CSB-YP427853DHW
CSB-EP427853DHW
CSB-BP427853DHW
CSB-MP427853DHW
CSB-EP427853DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP427854DHW
CSB-EP427854DHW
CSB-BP427854DHW
CSB-MP427854DHW
CSB-EP427854DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans DNA repair protein recO (recO) CSB-YP427855DHW
CSB-EP427855DHW
CSB-BP427855DHW
CSB-MP427855DHW
CSB-EP427855DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP427856DHW
CSB-EP427856DHW
CSB-BP427856DHW
CSB-MP427856DHW
CSB-EP427856DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus 50S ribosomal protein L2P (rpl2p) CSB-YP427857NFB
CSB-EP427857NFB
CSB-BP427857NFB
CSB-MP427857NFB
CSB-EP427857NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Probable porphobilinogen deaminase (hemC) CSB-YP427858NFB
CSB-EP427858NFB
CSB-BP427858NFB
CSB-MP427858NFB
CSB-EP427858NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus UPF0218 protein Nmar_1233 (Nmar_1233) CSB-YP427859NFB
CSB-EP427859NFB
CSB-BP427859NFB
CSB-MP427859NFB
CSB-EP427859NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>