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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rickettsia bellii ATP synthase gamma chain (atpG) CSB-YP426819RIM
CSB-EP426819RIM
CSB-BP426819RIM
CSB-MP426819RIM
CSB-EP426819RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP426820RIM
CSB-EP426820RIM
CSB-BP426820RIM
CSB-MP426820RIM
CSB-EP426820RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP426821RIM
CSB-EP426821RIM
CSB-BP426821RIM
CSB-MP426821RIM
CSB-EP426821RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Chromosomal replication initiator protein DnaA (dnaA) CSB-YP426822STP
CSB-EP426822STP
CSB-BP426822STP
CSB-MP426822STP
CSB-EP426822STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Glutamate racemase (murI) CSB-YP426823STP
CSB-EP426823STP
CSB-BP426823STP
CSB-MP426823STP
CSB-EP426823STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP426824STP
CSB-EP426824STP
CSB-BP426824STP
CSB-MP426824STP
CSB-EP426824STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L22 (rplV) CSB-YP426825STP
CSB-EP426825STP
CSB-BP426825STP
CSB-MP426825STP
CSB-EP426825STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L14 (rplN) CSB-YP426826STP
CSB-EP426826STP
CSB-BP426826STP
CSB-MP426826STP
CSB-EP426826STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 30S ribosomal protein S13 (rpsM) CSB-YP426827STP
CSB-EP426827STP
CSB-BP426827STP
CSB-MP426827STP
CSB-EP426827STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP426828STP
CSB-EP426828STP
CSB-BP426828STP
CSB-MP426828STP
CSB-EP426828STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Acetylglutamate kinase (argB) CSB-YP426829STP
CSB-EP426829STP
CSB-BP426829STP
CSB-MP426829STP
CSB-EP426829STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana ATP-dependent RNA helicase rhlB (rhlB) CSB-YP426830STP
CSB-EP426830STP
CSB-BP426830STP
CSB-MP426830STP
CSB-EP426830STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP426831STP
CSB-EP426831STP
CSB-BP426831STP
CSB-MP426831STP
CSB-EP426831STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Pantothenate synthetase (panC) CSB-YP426832STP
CSB-EP426832STP
CSB-BP426832STP
CSB-MP426832STP
CSB-EP426832STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Phosphoglycerate kinase (pgk) CSB-YP426833STP
CSB-EP426833STP
CSB-BP426833STP
CSB-MP426833STP
CSB-EP426833STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana GTPase obg (obg) CSB-YP426834STP
CSB-EP426834STP
CSB-BP426834STP
CSB-MP426834STP
CSB-EP426834STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP426835STP
CSB-EP426835STP
CSB-BP426835STP
CSB-MP426835STP
CSB-EP426835STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Elongation factor 4 (lepA), partial CSB-YP426836STP
CSB-EP426836STP
CSB-BP426836STP
CSB-MP426836STP
CSB-EP426836STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Isoleucine--tRNA ligase (ileS), partial CSB-YP426837STP
CSB-EP426837STP
CSB-BP426837STP
CSB-MP426837STP
CSB-EP426837STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP426838STP
CSB-EP426838STP
CSB-BP426838STP
CSB-MP426838STP
CSB-EP426838STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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