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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella pealeana tRNA pseudouridine synthase B (truB) CSB-YP426859STP
CSB-EP426859STP
CSB-BP426859STP
CSB-MP426859STP
CSB-EP426859STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Chaperone protein DnaJ (dnaJ) CSB-YP426860STP
CSB-EP426860STP
CSB-BP426860STP
CSB-MP426860STP
CSB-EP426860STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana DNA polymerase IV (dinB) CSB-YP426861STP
CSB-EP426861STP
CSB-BP426861STP
CSB-MP426861STP
CSB-EP426861STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP426862STP
CSB-EP426862STP
CSB-BP426862STP
CSB-MP426862STP
CSB-EP426862STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L9 (rplI) CSB-YP426863STP
CSB-EP426863STP
CSB-BP426863STP
CSB-MP426863STP
CSB-EP426863STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana UPF0251 protein Spea_3639 (Spea_3639) CSB-YP426864STP
CSB-EP426864STP
CSB-BP426864STP
CSB-MP426864STP
CSB-EP426864STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Formate--tetrahydrofolate ligase (fhs), partial CSB-YP426865STP
CSB-EP426865STP
CSB-BP426865STP
CSB-MP426865STP
CSB-EP426865STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Sec-independent protein translocase protein TatA (tatA), partial CSB-YP426866STP
CSB-EP426866STP
CSB-BP426866STP
CSB-MP426866STP
CSB-EP426866STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP426867STP
CSB-EP426867STP
CSB-BP426867STP
CSB-MP426867STP
CSB-EP426867STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 2-isopropylmalate synthase (leuA), partial CSB-YP426868STP
CSB-EP426868STP
CSB-BP426868STP
CSB-MP426868STP
CSB-EP426868STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP426869STP
CSB-EP426869STP
CSB-BP426869STP
CSB-MP426869STP
CSB-EP426869STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP426870STP
CSB-EP426870STP
CSB-BP426870STP
CSB-MP426870STP
CSB-EP426870STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Imidazolonepropionase (hutI) CSB-YP426871STP
CSB-EP426871STP
CSB-BP426871STP
CSB-MP426871STP
CSB-EP426871STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana ATP synthase epsilon chain (atpC) CSB-YP426872STP
CSB-EP426872STP
CSB-BP426872STP
CSB-MP426872STP
CSB-EP426872STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Membrane protein insertase YidC (yidC), partial CSB-YP426873STP1
CSB-EP426873STP1
CSB-BP426873STP1
CSB-MP426873STP1
CSB-EP426873STP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropidechis carinatus Short neurotoxin 1 CSB-YP426874TQQ
CSB-EP426874TQQ
CSB-BP426874TQQ
CSB-MP426874TQQ
CSB-EP426874TQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hoplocephalus stephensii Short neurotoxin 1 CSB-YP426875HWP
CSB-EP426875HWP
CSB-BP426875HWP
CSB-MP426875HWP
CSB-EP426875HWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyuranus microlepidotus Long neurotoxin 2 CSB-YP426876OGE
CSB-EP426876OGE
CSB-BP426876OGE
CSB-MP426876OGE
CSB-EP426876OGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP426877DPR
CSB-EP426877DPR
CSB-BP426877DPR
CSB-MP426877DPR
CSB-EP426877DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP426878DPR
CSB-EP426878DPR
CSB-BP426878DPR
CSB-MP426878DPR
CSB-EP426878DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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