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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coprinopsis cinerea Nucleolar protein 9 (NOP9), partial CSB-YP427060DWQ
CSB-EP427060DWQ
CSB-BP427060DWQ
CSB-MP427060DWQ
CSB-EP427060DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea 60S ribosomal protein L44 (RPL44) CSB-YP427061DWQ
CSB-EP427061DWQ
CSB-BP427061DWQ
CSB-MP427061DWQ
CSB-EP427061DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Fe-S cluster assembly protein DRE2 (DRE2) CSB-YP427062MNH
CSB-EP427062MNH
CSB-BP427062MNH
CSB-MP427062MNH
CSB-EP427062MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Succinate dehydrogenase assembly factor 1, mitochondrial (SDHAF1) CSB-YP427063BO
CSB-EP427063BO
CSB-BP427063BO
CSB-MP427063BO
CSB-EP427063BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa 40S ribosomal protein S0 (RPS0) CSB-YP427064MNH
CSB-EP427064MNH
CSB-BP427064MNH
CSB-MP427064MNH
CSB-EP427064MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa ATPase GET3 (GET3) CSB-YP427065MNH
CSB-EP427065MNH
CSB-BP427065MNH
CSB-MP427065MNH
CSB-EP427065MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi ATPase ASNA1 homolog (Bm1_42140) CSB-YP427066BWV
CSB-EP427066BWV
CSB-BP427066BWV
CSB-MP427066BWV
CSB-EP427066BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (MGL_3505), partial CSB-YP427067MNH
CSB-EP427067MNH
CSB-BP427067MNH
CSB-MP427067MNH
CSB-EP427067MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Adenylosuccinate synthetase (Bm1_50695) CSB-YP427068BWV
CSB-EP427068BWV
CSB-BP427068BWV
CSB-MP427068BWV
CSB-EP427068BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor 5-pentadecatrienyl resorcinol O-methyltransferase (OMT3) CSB-YP427069FJS
CSB-EP427069FJS
CSB-BP427069FJS
CSB-MP427069FJS
CSB-EP427069FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Austrelaps superbus Long neurotoxin 1 CSB-YP427070DPN
CSB-EP427070DPN
CSB-BP427070DPN
CSB-MP427070DPN
CSB-EP427070DPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Photosystem Q (B) protein, partial CSB-YP427071CWK1
CSB-EP427071CWK1
CSB-BP427071CWK1
CSB-MP427071CWK1
CSB-EP427071CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP427072CWK
CSB-EP427072CWK
CSB-BP427072CWK
CSB-MP427072CWK
CSB-EP427072CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP427073CWK
CSB-EP427073CWK
CSB-BP427073CWK
CSB-MP427073CWK
CSB-EP427073CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP427074CWK1
CSB-EP427074CWK1
CSB-BP427074CWK1
CSB-MP427074CWK1
CSB-EP427074CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP427075CWK
CSB-EP427075CWK
CSB-BP427075CWK
CSB-MP427075CWK
CSB-EP427075CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP427076CWK1
CSB-EP427076CWK1
CSB-BP427076CWK1
CSB-MP427076CWK1
CSB-EP427076CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP427077CWK
CSB-EP427077CWK
CSB-BP427077CWK
CSB-MP427077CWK
CSB-EP427077CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Protein ycf2 (ycf2-A), partial CSB-YP427078CWK
CSB-EP427078CWK
CSB-BP427078CWK
CSB-MP427078CWK
CSB-EP427078CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum NAD (P)H-quinone oxidoreductase subunit H, chloroplastic CSB-YP427079CWK
CSB-EP427079CWK
CSB-BP427079CWK
CSB-MP427079CWK
CSB-EP427079CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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