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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus helveticus 50S ribosomal protein L35 (rpmI) CSB-YP428260LLE
CSB-EP428260LLE
CSB-BP428260LLE
CSB-MP428260LLE
CSB-EP428260LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP428261LLE
CSB-EP428261LLE
CSB-BP428261LLE
CSB-MP428261LLE
CSB-EP428261LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Putative 3-methyladenine DNA glycosylase (lhv_0124) CSB-YP428262LLE
CSB-EP428262LLE
CSB-BP428262LLE
CSB-MP428262LLE
CSB-EP428262LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus S-adenosylmethionine synthase (metK) CSB-YP428263LLE
CSB-EP428263LLE
CSB-BP428263LLE
CSB-MP428263LLE
CSB-EP428263LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP428264LLE
CSB-EP428264LLE
CSB-BP428264LLE
CSB-MP428264LLE
CSB-EP428264LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Peptidyl-tRNA hydrolase (pth) CSB-YP428265LLE
CSB-EP428265LLE
CSB-BP428265LLE
CSB-MP428265LLE
CSB-EP428265LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S12 (rpsL) CSB-YP428266LLE
CSB-EP428266LLE
CSB-BP428266LLE
CSB-MP428266LLE
CSB-EP428266LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L3 (rplC) CSB-YP428267LLE
CSB-EP428267LLE
CSB-BP428267LLE
CSB-MP428267LLE
CSB-EP428267LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S19 (rpsS) CSB-YP428268LLE
CSB-EP428268LLE
CSB-BP428268LLE
CSB-MP428268LLE
CSB-EP428268LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L29 (rpmC) CSB-YP428269LLE
CSB-EP428269LLE
CSB-BP428269LLE
CSB-MP428269LLE
CSB-EP428269LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S8 (rpsH) CSB-YP428270LLE
CSB-EP428270LLE
CSB-BP428270LLE
CSB-MP428270LLE
CSB-EP428270LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L15 (rplO) CSB-YP428271LLE
CSB-EP428271LLE
CSB-BP428271LLE
CSB-MP428271LLE
CSB-EP428271LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S11 (rpsK) CSB-YP428272LLE
CSB-EP428272LLE
CSB-BP428272LLE
CSB-MP428272LLE
CSB-EP428272LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus S-ribosylhomocysteine lyase (luxS) CSB-YP428273LLE
CSB-EP428273LLE
CSB-BP428273LLE
CSB-MP428273LLE
CSB-EP428273LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0342 protein USA300HOU_1838 (USA300HOU_1838) CSB-YP428274SUM
CSB-EP428274SUM
CSB-BP428274SUM
CSB-MP428274SUM
CSB-EP428274SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0374 protein USA300HOU_1862 (USA300HOU_1862) CSB-YP428275SUM
CSB-EP428275SUM
CSB-BP428275SUM
CSB-MP428275SUM
CSB-EP428275SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase gamma chain (atpG) CSB-YP428276SUM
CSB-EP428276SUM
CSB-BP428276SUM
CSB-MP428276SUM
CSB-EP428276SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP428277SUM
CSB-EP428277SUM
CSB-BP428277SUM
CSB-MP428277SUM
CSB-EP428277SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoglucosamine mutase (glmM) CSB-YP428278SUM
CSB-EP428278SUM
CSB-BP428278SUM
CSB-MP428278SUM
CSB-EP428278SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tagatose 1,6-diphosphate aldolase (lacD) CSB-YP428279SUM
CSB-EP428279SUM
CSB-BP428279SUM
CSB-MP428279SUM
CSB-EP428279SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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