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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Alkaliphilus oremlandii Serine--tRNA ligase (serS) CSB-YP428460AUQ
CSB-EP428460AUQ
CSB-BP428460AUQ
CSB-MP428460AUQ
CSB-EP428460AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP428461AUQ
CSB-EP428461AUQ
CSB-BP428461AUQ
CSB-MP428461AUQ
CSB-EP428461AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP428462AUQ
CSB-EP428462AUQ
CSB-BP428462AUQ
CSB-MP428462AUQ
CSB-EP428462AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Dihydrodipicolinate reductase (dapB) CSB-YP428463AUQ
CSB-EP428463AUQ
CSB-BP428463AUQ
CSB-MP428463AUQ
CSB-EP428463AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii LexA repressor (lexA) CSB-YP428464AUQ
CSB-EP428464AUQ
CSB-BP428464AUQ
CSB-MP428464AUQ
CSB-EP428464AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP428465AUQ
CSB-EP428465AUQ
CSB-BP428465AUQ
CSB-MP428465AUQ
CSB-EP428465AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP428466AUQ
CSB-EP428466AUQ
CSB-BP428466AUQ
CSB-MP428466AUQ
CSB-EP428466AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii DNA repair protein recO (recO) CSB-YP428467AUQ
CSB-EP428467AUQ
CSB-BP428467AUQ
CSB-MP428467AUQ
CSB-EP428467AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Large-conductance mechanosensitive channel (mscL), partial CSB-YP428468AUQ1
CSB-EP428468AUQ1
CSB-BP428468AUQ1
CSB-MP428468AUQ1
CSB-EP428468AUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Deoxyribose-phosphate aldolase (deoC) CSB-YP428469AUQ
CSB-EP428469AUQ
CSB-BP428469AUQ
CSB-MP428469AUQ
CSB-EP428469AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Cell division protein SepF (sepF) CSB-YP428470AUQ
CSB-EP428470AUQ
CSB-BP428470AUQ
CSB-MP428470AUQ
CSB-EP428470AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Ribosome maturation factor RimM (rimM) CSB-YP428471AUQ
CSB-EP428471AUQ
CSB-BP428471AUQ
CSB-MP428471AUQ
CSB-EP428471AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Ribonuclease HII (rnhB) CSB-YP428472AUQ
CSB-EP428472AUQ
CSB-BP428472AUQ
CSB-MP428472AUQ
CSB-EP428472AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii GTPase obg (obg) CSB-YP428473AUQ
CSB-EP428473AUQ
CSB-BP428473AUQ
CSB-MP428473AUQ
CSB-EP428473AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 30S ribosomal protein S4 B (rpsD2) CSB-YP428474AUQ
CSB-EP428474AUQ
CSB-BP428474AUQ
CSB-MP428474AUQ
CSB-EP428474AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP428475AUQ
CSB-EP428475AUQ
CSB-BP428475AUQ
CSB-MP428475AUQ
CSB-EP428475AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Formate--tetrahydrofolate ligase (fhs), partial CSB-YP428476AUQ
CSB-EP428476AUQ
CSB-BP428476AUQ
CSB-MP428476AUQ
CSB-EP428476AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UvrABC system protein C (uvrC), partial CSB-YP428477AUQ
CSB-EP428477AUQ
CSB-BP428477AUQ
CSB-MP428477AUQ
CSB-EP428477AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Carbon storage regulator homolog (csrA) CSB-YP428478AUQ
CSB-EP428478AUQ
CSB-BP428478AUQ
CSB-MP428478AUQ
CSB-EP428478AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP428479AUQ
CSB-EP428479AUQ
CSB-BP428479AUQ
CSB-MP428479AUQ
CSB-EP428479AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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