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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Iron-sulfur cluster repair protein ScdA (scdA) CSB-YP428660SUM
CSB-EP428660SUM
CSB-BP428660SUM
CSB-MP428660SUM
CSB-EP428660SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-ribose pyranase (rbsD) CSB-YP428661SUM
CSB-EP428661SUM
CSB-BP428661SUM
CSB-MP428661SUM
CSB-EP428661SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phenol-soluble modulin alpha 1 peptide (psmA1) CSB-YP428662SUM
CSB-EP428662SUM
CSB-BP428662SUM
CSB-MP428662SUM
CSB-EP428662SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP428663SUM
CSB-EP428663SUM
CSB-BP428663SUM
CSB-MP428663SUM
CSB-EP428663SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginine--tRNA ligase (argS), partial CSB-YP428664SUM
CSB-EP428664SUM
CSB-BP428664SUM
CSB-MP428664SUM
CSB-EP428664SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhE2 (mnhE2), partial CSB-YP428665SUM1
CSB-EP428665SUM1
CSB-BP428665SUM1
CSB-MP428665SUM1
CSB-EP428665SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator protein graR (graR) CSB-YP428666SUM
CSB-EP428666SUM
CSB-BP428666SUM
CSB-MP428666SUM
CSB-EP428666SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Enolase (eno) CSB-YP428667SUM
CSB-EP428667SUM
CSB-BP428667SUM
CSB-MP428667SUM
CSB-EP428667SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional protein FolD (folD) CSB-YP428668SUM
CSB-EP428668SUM
CSB-BP428668SUM
CSB-MP428668SUM
CSB-EP428668SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0358 protein USA300HOU_1050 (USA300HOU_1050) CSB-YP428669SUM
CSB-EP428669SUM
CSB-BP428669SUM
CSB-MP428669SUM
CSB-EP428669SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0298 protein USA300HOU_1059 (USA300HOU_1059) CSB-YP428670SUM
CSB-EP428670SUM
CSB-BP428670SUM
CSB-MP428670SUM
CSB-EP428670SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein A (isdA) CSB-YP428671SUM
CSB-EP428671SUM
CSB-BP428671SUM
CSB-MP428671SUM
CSB-EP428671SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme-degrading monooxygenase isdG (isdG) CSB-YP428672SUM
CSB-EP428672SUM
CSB-BP428672SUM
CSB-MP428672SUM
CSB-EP428672SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate racemase (murI) CSB-YP428673SUM
CSB-EP428673SUM
CSB-BP428673SUM
CSB-MP428673SUM
CSB-EP428673SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP428674SUM
CSB-EP428674SUM
CSB-BP428674SUM
CSB-MP428674SUM
CSB-EP428674SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol kinase (glpK) CSB-YP428675SUM
CSB-EP428675SUM
CSB-BP428675SUM
CSB-MP428675SUM
CSB-EP428675SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP428676SUM1
CSB-EP428676SUM1
CSB-BP428676SUM1
CSB-MP428676SUM1
CSB-EP428676SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease Z (rnz) CSB-YP428677SUM
CSB-EP428677SUM
CSB-BP428677SUM
CSB-MP428677SUM
CSB-EP428677SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0735 ACT domain-containing protein USA300HOU_1642 (USA300HOU_1642) CSB-YP428678SUM
CSB-EP428678SUM
CSB-BP428678SUM
CSB-MP428678SUM
CSB-EP428678SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L21 (rplU) CSB-YP428679SUM
CSB-EP428679SUM
CSB-BP428679SUM
CSB-MP428679SUM
CSB-EP428679SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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