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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coprinopsis cinerea Histone H2A.Z-specific chaperone CHZ1 (CHZ1) CSB-YP427420DWQ
CSB-EP427420DWQ
CSB-BP427420DWQ
CSB-MP427420DWQ
CSB-EP427420DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Tryptophan synthase (TRP-1), partial CSB-YP427421DWQ
CSB-EP427421DWQ
CSB-BP427421DWQ
CSB-MP427421DWQ
CSB-EP427421DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein C2orf81 homolog, partial CSB-YP427422BO
CSB-EP427422BO
CSB-BP427422BO
CSB-MP427422BO
CSB-EP427422BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea 24 kDa Ras-like protein (CC-RAS) CSB-YP427423DWQ
CSB-EP427423DWQ
CSB-BP427423DWQ
CSB-MP427423DWQ
CSB-EP427423DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Ribosome biogenesis protein ERB1 (ERB1), partial CSB-YP427424DWQ
CSB-EP427424DWQ
CSB-BP427424DWQ
CSB-MP427424DWQ
CSB-EP427424DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea tRNA-dihydrouridine (47) synthase [NAD (P) (+)], partial CSB-YP427425DWQ
CSB-EP427425DWQ
CSB-BP427425DWQ
CSB-MP427425DWQ
CSB-EP427425DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Ubiquinone biosynthesis protein COQ4, mitochondrial (COQ4) CSB-YP427426DWQ
CSB-EP427426DWQ
CSB-BP427426DWQ
CSB-MP427426DWQ
CSB-EP427426DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Protein CLP1 homolog (Bm1_20975) CSB-YP427427BWV
CSB-EP427427BWV
CSB-BP427427BWV
CSB-MP427427BWV
CSB-EP427427BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Probable cytosolic Fe-S cluster assembly factor Bm1_25010 (Bm1_25010) CSB-YP427428BWV
CSB-EP427428BWV
CSB-BP427428BWV
CSB-MP427428BWV
CSB-EP427428BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Mitochondrial division protein 1 (MDV1), partial CSB-YP427429MNH
CSB-EP427429MNH
CSB-BP427429MNH
CSB-MP427429MNH
CSB-EP427429MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Spindle and kinetochore-associated protein 3 (SKA3) CSB-YP427430BO
CSB-EP427430BO
CSB-BP427430BO
CSB-MP427430BO
CSB-EP427430BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Elongation factor G, mitochondrial (MEF1), partial CSB-YP427431MNH
CSB-EP427431MNH
CSB-BP427431MNH
CSB-MP427431MNH
CSB-EP427431MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Translation factor GUF1 homolog, mitochondrial (Bm1_49530), partial CSB-YP427432BWV
CSB-EP427432BWV
CSB-BP427432BWV
CSB-MP427432BWV
CSB-EP427432BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa 40S ribosomal protein S1 (RPS1) CSB-YP427433MNH
CSB-EP427433MNH
CSB-BP427433MNH
CSB-MP427433MNH
CSB-EP427433MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Probable spastin homolog Bm1_53365 (Bm1_53365) CSB-YP427434BWV
CSB-EP427434BWV
CSB-BP427434BWV
CSB-MP427434BWV
CSB-EP427434BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Austrelaps superbus Long neurotoxin 2 CSB-YP427435DPN
CSB-EP427435DPN
CSB-BP427435DPN
CSB-MP427435DPN
CSB-EP427435DPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Photosystem II reaction center protein I (psbI), partial CSB-YP427436CWK
CSB-EP427436CWK
CSB-BP427436CWK
CSB-MP427436CWK
CSB-EP427436CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP427437CWK1
CSB-EP427437CWK1
CSB-BP427437CWK1
CSB-MP427437CWK1
CSB-EP427437CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP427438CWK
CSB-EP427438CWK
CSB-BP427438CWK
CSB-MP427438CWK
CSB-EP427438CWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratophyllum demersum Apocytochrome f (petA), partial CSB-YP427439CWK1
CSB-EP427439CWK1
CSB-BP427439CWK1
CSB-MP427439CWK1
CSB-EP427439CWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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