Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lolium perenne 30S ribosomal protein S15, chloroplastic (rps15-A) CSB-YP428220LQK
CSB-EP428220LQK
CSB-BP428220LQK
CSB-MP428220LQK
CSB-EP428220LQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lolium perenne NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP428221LQK1
CSB-EP428221LQK1
CSB-BP428221LQK1
CSB-MP428221LQK1
CSB-EP428221LQK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lolium perenne Photosystem II D2 protein (psbD), partial CSB-YP428222LQK1
CSB-EP428222LQK1
CSB-BP428222LQK1
CSB-MP428222LQK1
CSB-EP428222LQK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lolium perenne ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP428223LQK1
CSB-EP428223LQK1
CSB-BP428223LQK1
CSB-MP428223LQK1
CSB-EP428223LQK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lolium perenne NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP428224LQK1
CSB-EP428224LQK1
CSB-BP428224LQK1
CSB-MP428224LQK1
CSB-EP428224LQK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP428225MOA
CSB-EP428225MOA
CSB-BP428225MOA
CSB-MP428225MOA
CSB-EP428225MOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Glutamate--tRNA ligase (gltX) CSB-YP428226LLE
CSB-EP428226LLE
CSB-BP428226LLE
CSB-MP428226LLE
CSB-EP428226LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L10 (rplJ) CSB-YP428227LLE
CSB-EP428227LLE
CSB-BP428227LLE
CSB-MP428227LLE
CSB-EP428227LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Recombination protein RecR (recR) CSB-YP428228LLE
CSB-EP428228LLE
CSB-BP428228LLE
CSB-MP428228LLE
CSB-EP428228LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus DNA mismatch repair protein mutL (mutL), partial CSB-YP428229LLE
CSB-EP428229LLE
CSB-BP428229LLE
CSB-MP428229LLE
CSB-EP428229LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus MutS2 protein (mutS2), partial CSB-YP428230LLE
CSB-EP428230LLE
CSB-BP428230LLE
CSB-MP428230LLE
CSB-EP428230LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP428231LLE
CSB-EP428231LLE
CSB-BP428231LLE
CSB-MP428231LLE
CSB-EP428231LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus tRNA modification GTPase MnmE (mnmE) CSB-YP428232LLE
CSB-EP428232LLE
CSB-BP428232LLE
CSB-MP428232LLE
CSB-EP428232LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Ferredoxin--NADP reductase (lhv_0465) CSB-YP428233LLE
CSB-EP428233LLE
CSB-BP428233LLE
CSB-MP428233LLE
CSB-EP428233LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Uridine kinase (udk) CSB-YP428234LLE
CSB-EP428234LLE
CSB-BP428234LLE
CSB-MP428234LLE
CSB-EP428234LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Peptide chain release factor 3 (prfC), partial CSB-YP428235LLE
CSB-EP428235LLE
CSB-BP428235LLE
CSB-MP428235LLE
CSB-EP428235LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Phosphoglycerate kinase (pgk) CSB-YP428236LLE
CSB-EP428236LLE
CSB-BP428236LLE
CSB-MP428236LLE
CSB-EP428236LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Protein MraZ (mraZ) CSB-YP428237LLE
CSB-EP428237LLE
CSB-BP428237LLE
CSB-MP428237LLE
CSB-EP428237LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP428238LLE
CSB-EP428238LLE
CSB-BP428238LLE
CSB-MP428238LLE
CSB-EP428238LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP428239LLE
CSB-EP428239LLE
CSB-BP428239LLE
CSB-MP428239LLE
CSB-EP428239LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>