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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Alkaliphilus oremlandii Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP427380AUQ
CSB-EP427380AUQ
CSB-BP427380AUQ
CSB-MP427380AUQ
CSB-EP427380AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Gamma-glutamyl phosphate reductase (proA) CSB-YP427381AUQ
CSB-EP427381AUQ
CSB-BP427381AUQ
CSB-MP427381AUQ
CSB-EP427381AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Ribosomal protein L11 methyltransferase (prmA) CSB-YP427382AUQ
CSB-EP427382AUQ
CSB-BP427382AUQ
CSB-MP427382AUQ
CSB-EP427382AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii DNA polymerase IV (dinB) CSB-YP427383AUQ
CSB-EP427383AUQ
CSB-BP427383AUQ
CSB-MP427383AUQ
CSB-EP427383AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Uncharacterized methyltransferase Clos_1076 (Clos_1076) CSB-YP427384AUQ
CSB-EP427384AUQ
CSB-BP427384AUQ
CSB-MP427384AUQ
CSB-EP427384AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP427385AUQ
CSB-EP427385AUQ
CSB-BP427385AUQ
CSB-MP427385AUQ
CSB-EP427385AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Phosphate acyltransferase (plsX) CSB-YP427386AUQ
CSB-EP427386AUQ
CSB-BP427386AUQ
CSB-MP427386AUQ
CSB-EP427386AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP427387AUQ
CSB-EP427387AUQ
CSB-BP427387AUQ
CSB-MP427387AUQ
CSB-EP427387AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UPF0758 protein Clos_1766 (Clos_1766) CSB-YP427388AUQ
CSB-EP427388AUQ
CSB-BP427388AUQ
CSB-MP427388AUQ
CSB-EP427388AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Homoserine kinase (thrB) CSB-YP427389AUQ
CSB-EP427389AUQ
CSB-BP427389AUQ
CSB-MP427389AUQ
CSB-EP427389AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UPF0234 protein Clos_1967 (Clos_1967) CSB-YP427390AUQ
CSB-EP427390AUQ
CSB-BP427390AUQ
CSB-MP427390AUQ
CSB-EP427390AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Trigger factor (tig) CSB-YP427391AUQ
CSB-EP427391AUQ
CSB-BP427391AUQ
CSB-MP427391AUQ
CSB-EP427391AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UPF0371 protein Clos_2302 (Clos_2302), partial CSB-YP427392AUQ
CSB-EP427392AUQ
CSB-BP427392AUQ
CSB-MP427392AUQ
CSB-EP427392AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii S-adenosylmethionine synthase (metK) CSB-YP427393AUQ
CSB-EP427393AUQ
CSB-BP427393AUQ
CSB-MP427393AUQ
CSB-EP427393AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Peptidyl-tRNA hydrolase (pth) CSB-YP427394AUQ
CSB-EP427394AUQ
CSB-BP427394AUQ
CSB-MP427394AUQ
CSB-EP427394AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Sugar fermentation stimulation protein homolog (sfsA) CSB-YP427395AUQ
CSB-EP427395AUQ
CSB-BP427395AUQ
CSB-MP427395AUQ
CSB-EP427395AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UPF0133 protein Clos_2855 (Clos_2855) CSB-YP427396AUQ
CSB-EP427396AUQ
CSB-BP427396AUQ
CSB-MP427396AUQ
CSB-EP427396AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L34 (rpmH) CSB-YP427397AUQ
CSB-EP427397AUQ
CSB-BP427397AUQ
CSB-MP427397AUQ
CSB-EP427397AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP427398AUQ
CSB-EP427398AUQ
CSB-BP427398AUQ
CSB-MP427398AUQ
CSB-EP427398AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L10 (rplJ) CSB-YP427399AUQ
CSB-EP427399AUQ
CSB-BP427399AUQ
CSB-MP427399AUQ
CSB-EP427399AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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