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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus helveticus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP428620LLE
CSB-EP428620LLE
CSB-BP428620LLE
CSB-MP428620LLE
CSB-EP428620LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UPF0348 protein lhv_1590 (lhv_1590) CSB-YP428621LLE
CSB-EP428621LLE
CSB-BP428621LLE
CSB-MP428621LLE
CSB-EP428621LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP428622LLE
CSB-EP428622LLE
CSB-BP428622LLE
CSB-MP428622LLE
CSB-EP428622LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Proline iminopeptidase (pip) CSB-YP428623LLE
CSB-EP428623LLE
CSB-BP428623LLE
CSB-MP428623LLE
CSB-EP428623LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UPF0342 protein lhv_1666 (lhv_1666) CSB-YP428624LLE
CSB-EP428624LLE
CSB-BP428624LLE
CSB-MP428624LLE
CSB-EP428624LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP428625LLE
CSB-EP428625LLE
CSB-BP428625LLE
CSB-MP428625LLE
CSB-EP428625LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP428626LLE
CSB-EP428626LLE
CSB-BP428626LLE
CSB-MP428626LLE
CSB-EP428626LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L31 type B (rpmE2) CSB-YP428627LLE
CSB-EP428627LLE
CSB-BP428627LLE
CSB-MP428627LLE
CSB-EP428627LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 33 kDa chaperonin (hslO) CSB-YP428628LLE
CSB-EP428628LLE
CSB-BP428628LLE
CSB-MP428628LLE
CSB-EP428628LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S10 (rpsJ) CSB-YP428629LLE
CSB-EP428629LLE
CSB-BP428629LLE
CSB-MP428629LLE
CSB-EP428629LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L2 (rplB) CSB-YP428630LLE
CSB-EP428630LLE
CSB-BP428630LLE
CSB-MP428630LLE
CSB-EP428630LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S3 (rpsC) CSB-YP428631LLE
CSB-EP428631LLE
CSB-BP428631LLE
CSB-MP428631LLE
CSB-EP428631LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L14 (rplN) CSB-YP428632LLE
CSB-EP428632LLE
CSB-BP428632LLE
CSB-MP428632LLE
CSB-EP428632LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S5 (rpsE) CSB-YP428633LLE
CSB-EP428633LLE
CSB-BP428633LLE
CSB-MP428633LLE
CSB-EP428633LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Translation initiation factor IF-1 (infA) CSB-YP428634LLE
CSB-EP428634LLE
CSB-BP428634LLE
CSB-MP428634LLE
CSB-EP428634LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L17 (rplQ) CSB-YP428635LLE
CSB-EP428635LLE
CSB-BP428635LLE
CSB-MP428635LLE
CSB-EP428635LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP428636LLE
CSB-EP428636LLE
CSB-BP428636LLE
CSB-MP428636LLE
CSB-EP428636LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Regulatory protein recX (recX) CSB-YP428637SUM
CSB-EP428637SUM
CSB-BP428637SUM
CSB-MP428637SUM
CSB-EP428637SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit c (atpE), partial CSB-YP428638SUM1
CSB-EP428638SUM1
CSB-BP428638SUM1
CSB-MP428638SUM1
CSB-EP428638SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L31 type B (rpmE2) CSB-YP428639SUM
CSB-EP428639SUM
CSB-BP428639SUM
CSB-MP428639SUM
CSB-EP428639SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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