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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Delftia acidovorans 50S ribosomal protein L5 (rplE) CSB-YP430021DHV
CSB-EP430021DHV
CSB-BP430021DHV
CSB-MP430021DHV
CSB-EP430021DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L6 (rplF) CSB-YP430022DHV
CSB-EP430022DHV
CSB-BP430022DHV
CSB-MP430022DHV
CSB-EP430022DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 30S ribosomal protein S11 (rpsK) CSB-YP430023DHV
CSB-EP430023DHV
CSB-BP430023DHV
CSB-MP430023DHV
CSB-EP430023DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Urease accessory protein UreG (ureG) CSB-YP430024DHV
CSB-EP430024DHV
CSB-BP430024DHV
CSB-MP430024DHV
CSB-EP430024DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans D-tyrosyl-tRNA (Tyr) deacylase CSB-YP430025DHV
CSB-EP430025DHV
CSB-BP430025DHV
CSB-MP430025DHV
CSB-EP430025DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP430026DHV
CSB-EP430026DHV
CSB-BP430026DHV
CSB-MP430026DHV
CSB-EP430026DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans LexA repressor (lexA) CSB-YP430027DHV
CSB-EP430027DHV
CSB-BP430027DHV
CSB-MP430027DHV
CSB-EP430027DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP430028DHV
CSB-EP430028DHV
CSB-BP430028DHV
CSB-MP430028DHV
CSB-EP430028DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP430029DHV
CSB-EP430029DHV
CSB-BP430029DHV
CSB-MP430029DHV
CSB-EP430029DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Serine--tRNA ligase (serS) CSB-YP430030DHV
CSB-EP430030DHV
CSB-BP430030DHV
CSB-MP430030DHV
CSB-EP430030DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP430031DHV
CSB-EP430031DHV
CSB-BP430031DHV
CSB-MP430031DHV
CSB-EP430031DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L13 (rplM) CSB-YP430032DHV
CSB-EP430032DHV
CSB-BP430032DHV
CSB-MP430032DHV
CSB-EP430032DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans FMN-dependent NADH-azoreductase (azoR) CSB-YP430033DHV
CSB-EP430033DHV
CSB-BP430033DHV
CSB-MP430033DHV
CSB-EP430033DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Probable Fe (2+)-trafficking protein CSB-YP430034DHV
CSB-EP430034DHV
CSB-BP430034DHV
CSB-MP430034DHV
CSB-EP430034DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Signal recognition particle receptor FtsY (ftsY) CSB-YP430035AYS
CSB-EP430035AYS
CSB-BP430035AYS
CSB-MP430035AYS
CSB-EP430035AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP430036AYS
CSB-EP430036AYS
CSB-BP430036AYS
CSB-MP430036AYS
CSB-EP430036AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP430037AYS
CSB-EP430037AYS
CSB-BP430037AYS
CSB-MP430037AYS
CSB-EP430037AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP430038AYS
CSB-EP430038AYS
CSB-BP430038AYS
CSB-MP430038AYS
CSB-EP430038AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterocytozoon bieneusi Probable U6 snRNA-associated Sm-like protein LSm2 (LSM2) CSB-YP430039EMA
CSB-EP430039EMA
CSB-BP430039EMA
CSB-MP430039EMA
CSB-EP430039EMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L20 (rplT) CSB-YP430040SUD
CSB-EP430040SUD
CSB-BP430040SUD
CSB-MP430040SUD
CSB-EP430040SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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