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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus helveticus 30S ribosomal protein S4 (rpsD) CSB-YP428600LLE
CSB-EP428600LLE
CSB-BP428600LLE
CSB-MP428600LLE
CSB-EP428600LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP428601LLE
CSB-EP428601LLE
CSB-BP428601LLE
CSB-MP428601LLE
CSB-EP428601LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UPF0356 protein lhv_0877 (lhv_0877) CSB-YP428602LLE
CSB-EP428602LLE
CSB-BP428602LLE
CSB-MP428602LLE
CSB-EP428602LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Probable GTP-binding protein EngB (engB) CSB-YP428603LLE
CSB-EP428603LLE
CSB-BP428603LLE
CSB-MP428603LLE
CSB-EP428603LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Dihydrodipicolinate synthase (dapA) CSB-YP428604LLE
CSB-EP428604LLE
CSB-BP428604LLE
CSB-MP428604LLE
CSB-EP428604LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UPF0756 membrane protein lhv_0995 (lhv_0995), partial CSB-YP428605LLE1
CSB-EP428605LLE1
CSB-BP428605LLE1
CSB-MP428605LLE1
CSB-EP428605LLE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UvrABC system protein C (uvrC), partial CSB-YP428606LLE
CSB-EP428606LLE
CSB-BP428606LLE
CSB-MP428606LLE
CSB-EP428606LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 50S ribosomal protein L32 (rpmF) CSB-YP428607LLE
CSB-EP428607LLE
CSB-BP428607LLE
CSB-MP428607LLE
CSB-EP428607LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UPF0346 protein lhv_1069 (lhv_1069) CSB-YP428608LLE
CSB-EP428608LLE
CSB-BP428608LLE
CSB-MP428608LLE
CSB-EP428608LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP428609LLE
CSB-EP428609LLE
CSB-BP428609LLE
CSB-MP428609LLE
CSB-EP428609LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP428610LLE
CSB-EP428610LLE
CSB-BP428610LLE
CSB-MP428610LLE
CSB-EP428610LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S21 (rpsU) CSB-YP428611LLE
CSB-EP428611LLE
CSB-BP428611LLE
CSB-MP428611LLE
CSB-EP428611LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Elongation factor 4 (lepA), partial CSB-YP428612LLE
CSB-EP428612LLE
CSB-BP428612LLE
CSB-MP428612LLE
CSB-EP428612LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus UPF0154 protein lhv_1362 (lhv_1362), partial CSB-YP428613LLE
CSB-EP428613LLE
CSB-BP428613LLE
CSB-MP428613LLE
CSB-EP428613LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S16 (rpsP) CSB-YP428614LLE
CSB-EP428614LLE
CSB-BP428614LLE
CSB-MP428614LLE
CSB-EP428614LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP428615LLE
CSB-EP428615LLE
CSB-BP428615LLE
CSB-MP428615LLE
CSB-EP428615LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Bifunctional protein FolD (folD1) CSB-YP428616LLE
CSB-EP428616LLE
CSB-BP428616LLE
CSB-MP428616LLE
CSB-EP428616LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Xaa-Pro dipeptidyl-peptidase (pepX), partial CSB-YP428617LLE
CSB-EP428617LLE
CSB-BP428617LLE
CSB-MP428617LLE
CSB-EP428617LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Dihydroorotase (pyrC) CSB-YP428618LLE
CSB-EP428618LLE
CSB-BP428618LLE
CSB-MP428618LLE
CSB-EP428618LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus 30S ribosomal protein S18 (rpsR) CSB-YP428619LLE
CSB-EP428619LLE
CSB-BP428619LLE
CSB-MP428619LLE
CSB-EP428619LLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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