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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfococcus oleovorans ATP phosphoribosyltransferase (hisG) CSB-YP428780DHW
CSB-EP428780DHW
CSB-BP428780DHW
CSB-MP428780DHW
CSB-EP428780DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Phosphopantetheine adenylyltransferase (coaD) CSB-YP428781DHW
CSB-EP428781DHW
CSB-BP428781DHW
CSB-MP428781DHW
CSB-EP428781DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Serine--tRNA ligase (serS) CSB-YP428782DHW
CSB-EP428782DHW
CSB-BP428782DHW
CSB-MP428782DHW
CSB-EP428782DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP428783DHW
CSB-EP428783DHW
CSB-BP428783DHW
CSB-MP428783DHW
CSB-EP428783DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Protein MraZ (mraZ) CSB-YP428784DHW
CSB-EP428784DHW
CSB-BP428784DHW
CSB-MP428784DHW
CSB-EP428784DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Alanine--tRNA ligase (alaS), partial CSB-YP428785DHW
CSB-EP428785DHW
CSB-BP428785DHW
CSB-MP428785DHW
CSB-EP428785DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP428786DHW
CSB-EP428786DHW
CSB-BP428786DHW
CSB-MP428786DHW
CSB-EP428786DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Ribosome maturation factor RimP (rimP) CSB-YP428787DHW
CSB-EP428787DHW
CSB-BP428787DHW
CSB-MP428787DHW
CSB-EP428787DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Formate--tetrahydrofolate ligase (fhs), partial CSB-YP428788DHW
CSB-EP428788DHW
CSB-BP428788DHW
CSB-MP428788DHW
CSB-EP428788DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Indole-3-glycerol phosphate synthase (trpC) CSB-YP428789DHW
CSB-EP428789DHW
CSB-BP428789DHW
CSB-MP428789DHW
CSB-EP428789DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP428790DHW
CSB-EP428790DHW
CSB-BP428790DHW
CSB-MP428790DHW
CSB-EP428790DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Threonine--tRNA ligase (thrS), partial CSB-YP428791DHW
CSB-EP428791DHW
CSB-BP428791DHW
CSB-MP428791DHW
CSB-EP428791DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP428792DHW
CSB-EP428792DHW
CSB-BP428792DHW
CSB-MP428792DHW
CSB-EP428792DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP428793DHW
CSB-EP428793DHW
CSB-BP428793DHW
CSB-MP428793DHW
CSB-EP428793DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans DNA ligase (ligA), partial CSB-YP428794DHW
CSB-EP428794DHW
CSB-BP428794DHW
CSB-MP428794DHW
CSB-EP428794DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP428795NFB
CSB-EP428795NFB
CSB-BP428795NFB
CSB-MP428795NFB
CSB-EP428795NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus 30S ribosomal protein S27ae (rps27Ae) CSB-YP428796NFB
CSB-EP428796NFB
CSB-BP428796NFB
CSB-MP428796NFB
CSB-EP428796NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Ketol-acid reductoisomerase (ilvC) CSB-YP428797NFB
CSB-EP428797NFB
CSB-BP428797NFB
CSB-MP428797NFB
CSB-EP428797NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus V-type ATP synthase beta chain (atpB) CSB-YP428798NFB
CSB-EP428798NFB
CSB-BP428798NFB
CSB-MP428798NFB
CSB-EP428798NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Proteasome subunit beta 2 (psmB2) CSB-YP428799NFB
CSB-EP428799NFB
CSB-BP428799NFB
CSB-MP428799NFB
CSB-EP428799NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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