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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L35 (rpmI) CSB-YP428840AUQ
CSB-EP428840AUQ
CSB-BP428840AUQ
CSB-MP428840AUQ
CSB-EP428840AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Probable GTP-binding protein EngB (engB) CSB-YP428841AUQ
CSB-EP428841AUQ
CSB-BP428841AUQ
CSB-MP428841AUQ
CSB-EP428841AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP428842AUQ
CSB-EP428842AUQ
CSB-BP428842AUQ
CSB-MP428842AUQ
CSB-EP428842AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Pyrrolidone-carboxylate peptidase (pcp) CSB-YP428843AUQ
CSB-EP428843AUQ
CSB-BP428843AUQ
CSB-MP428843AUQ
CSB-EP428843AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii ATP synthase epsilon chain (atpC) CSB-YP428844AUQ
CSB-EP428844AUQ
CSB-BP428844AUQ
CSB-MP428844AUQ
CSB-EP428844AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Hydroxyethylthiazole kinase (thiM) CSB-YP428845AUQ
CSB-EP428845AUQ
CSB-BP428845AUQ
CSB-MP428845AUQ
CSB-EP428845AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L9 (rplI) CSB-YP428846AUQ
CSB-EP428846AUQ
CSB-BP428846AUQ
CSB-MP428846AUQ
CSB-EP428846AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii tRNA modification GTPase MnmE (mnmE) CSB-YP428847AUQ
CSB-EP428847AUQ
CSB-BP428847AUQ
CSB-MP428847AUQ
CSB-EP428847AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP428848AUQ
CSB-EP428848AUQ
CSB-BP428848AUQ
CSB-MP428848AUQ
CSB-EP428848AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 30S ribosomal protein S10 (rpsJ) CSB-YP428849AUQ
CSB-EP428849AUQ
CSB-BP428849AUQ
CSB-MP428849AUQ
CSB-EP428849AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L16 (rplP) CSB-YP428850AUQ
CSB-EP428850AUQ
CSB-BP428850AUQ
CSB-MP428850AUQ
CSB-EP428850AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 50S ribosomal protein L30 (rpmD) CSB-YP428851AUQ
CSB-EP428851AUQ
CSB-BP428851AUQ
CSB-MP428851AUQ
CSB-EP428851AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii 30S ribosomal protein S9 (rpsI) CSB-YP428852AUQ
CSB-EP428852AUQ
CSB-BP428852AUQ
CSB-MP428852AUQ
CSB-EP428852AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP428853AUQ
CSB-EP428853AUQ
CSB-BP428853AUQ
CSB-MP428853AUQ
CSB-EP428853AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein ENSP00000344348 CSB-YP428854HU
CSB-EP428854HU
CSB-BP428854HU
CSB-MP428854HU
CSB-EP428854HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Non-specific lipid-transfer protein 13 (LTP13) CSB-YP428855DOA
CSB-EP428855DOA
CSB-BP428855DOA
CSB-MP428855DOA
CSB-EP428855DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana WPP domain-interacting tail-anchored protein 2 (WIT2), partial CSB-YP428856DOA1
CSB-EP428856DOA1
CSB-BP428856DOA1
CSB-MP428856DOA1
CSB-EP428856DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 200 (At5g59105) CSB-YP428857DOA
CSB-EP428857DOA
CSB-BP428857DOA
CSB-MP428857DOA
CSB-EP428857DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Carbon catabolite repressor protein 4 homolog 4 (CCR4-4) CSB-YP428858DOA
CSB-EP428858DOA
CSB-BP428858DOA
CSB-MP428858DOA
CSB-EP428858DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative PDZ domain-containing protein 1P (PDZK1P1) CSB-YP428859HU
CSB-EP428859HU
CSB-BP428859HU
CSB-MP428859HU
CSB-EP428859HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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