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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia multivorans Chorismate synthase (aroC) CSB-YP428980BPS
CSB-EP428980BPS
CSB-BP428980BPS
CSB-MP428980BPS
CSB-EP428980BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ATP-dependent protease subunit HslV (hslV) CSB-YP428981BPS
CSB-EP428981BPS
CSB-BP428981BPS
CSB-MP428981BPS
CSB-EP428981BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Polyphosphate kinase (ppk), partial CSB-YP428982BPS
CSB-EP428982BPS
CSB-BP428982BPS
CSB-MP428982BPS
CSB-EP428982BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Protein phosphatase CheZ (cheZ) CSB-YP428983BPS
CSB-EP428983BPS
CSB-BP428983BPS
CSB-MP428983BPS
CSB-EP428983BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L32 (rpmF) CSB-YP428984BPS
CSB-EP428984BPS
CSB-BP428984BPS
CSB-MP428984BPS
CSB-EP428984BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S7 (rpsG) CSB-YP428985BPS
CSB-EP428985BPS
CSB-BP428985BPS
CSB-MP428985BPS
CSB-EP428985BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L22 (rplV) CSB-YP428986BPS
CSB-EP428986BPS
CSB-BP428986BPS
CSB-MP428986BPS
CSB-EP428986BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L6 (rplF) CSB-YP428987BPS
CSB-EP428987BPS
CSB-BP428987BPS
CSB-MP428987BPS
CSB-EP428987BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L17 (rplQ) CSB-YP428988BPS
CSB-EP428988BPS
CSB-BP428988BPS
CSB-MP428988BPS
CSB-EP428988BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans tRNA (guanine-N (1)-)-methyltransferase CSB-YP428989BPS
CSB-EP428989BPS
CSB-BP428989BPS
CSB-MP428989BPS
CSB-EP428989BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Sec-independent protein translocase protein TatA (tatA), partial CSB-YP428990BPS
CSB-EP428990BPS
CSB-BP428990BPS
CSB-MP428990BPS
CSB-EP428990BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Arginine--tRNA ligase (argS), partial CSB-YP428991BPS
CSB-EP428991BPS
CSB-BP428991BPS
CSB-MP428991BPS
CSB-EP428991BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ribonuclease PH (rph) CSB-YP428992BPS
CSB-EP428992BPS
CSB-BP428992BPS
CSB-MP428992BPS
CSB-EP428992BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP428993BPS
CSB-EP428993BPS
CSB-BP428993BPS
CSB-MP428993BPS
CSB-EP428993BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Urease subunit gamma (ureA) CSB-YP428994BPS
CSB-EP428994BPS
CSB-BP428994BPS
CSB-MP428994BPS
CSB-EP428994BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Aspartate carbamoyltransferase (pyrB) CSB-YP428995BPS
CSB-EP428995BPS
CSB-BP428995BPS
CSB-MP428995BPS
CSB-EP428995BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Homogentisate 1,2-dioxygenase (hmgA) CSB-YP428996BPS
CSB-EP428996BPS
CSB-BP428996BPS
CSB-MP428996BPS
CSB-EP428996BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP428997BPS
CSB-EP428997BPS
CSB-BP428997BPS
CSB-MP428997BPS
CSB-EP428997BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP428998BPS
CSB-EP428998BPS
CSB-BP428998BPS
CSB-MP428998BPS
CSB-EP428998BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Kynurenine formamidase (kynB) CSB-YP428999BPS
CSB-EP428999BPS
CSB-BP428999BPS
CSB-MP428999BPS
CSB-EP428999BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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