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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP430662BON
CSB-EP430662BON
CSB-BP430662BON
CSB-MP430662BON
CSB-EP430662BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Metallothiol transferase fosB (fosB) CSB-YP430663BON
CSB-EP430663BON
CSB-BP430663BON
CSB-MP430663BON
CSB-EP430663BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Protein RecA (recA) CSB-YP430664BON
CSB-EP430664BON
CSB-BP430664BON
CSB-MP430664BON
CSB-EP430664BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP430665BON
CSB-EP430665BON
CSB-BP430665BON
CSB-MP430665BON
CSB-EP430665BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0295 protein BcerKBAB4_0454 (BcerKBAB4_0454), partial CSB-YP430666BON1
CSB-EP430666BON1
CSB-BP430666BON1
CSB-MP430666BON1
CSB-EP430666BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Proline--tRNA ligase (proS), partial CSB-YP430667BON
CSB-EP430667BON
CSB-BP430667BON
CSB-MP430667BON
CSB-EP430667BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP-dependent protease subunit HslV (hslV) CSB-YP430668BON
CSB-EP430668BON
CSB-BP430668BON
CSB-MP430668BON
CSB-EP430668BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribosome maturation factor RimM (rimM) CSB-YP430669BON
CSB-EP430669BON
CSB-BP430669BON
CSB-MP430669BON
CSB-EP430669BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP430670BON
CSB-EP430670BON
CSB-BP430670BON
CSB-MP430670BON
CSB-EP430670BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP430671BON
CSB-EP430671BON
CSB-BP430671BON
CSB-MP430671BON
CSB-EP430671BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribonuclease P protein component (rnpA) CSB-YP430672BON
CSB-EP430672BON
CSB-BP430672BON
CSB-MP430672BON
CSB-EP430672BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0346 protein BcerKBAB4_2120 (BcerKBAB4_2120) CSB-YP430673BON
CSB-EP430673BON
CSB-BP430673BON
CSB-MP430673BON
CSB-EP430673BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L32 (rpmF) CSB-YP430674BON
CSB-EP430674BON
CSB-BP430674BON
CSB-MP430674BON
CSB-EP430674BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Heme A synthase (ctaA), partial CSB-YP430675BON
CSB-EP430675BON
CSB-BP430675BON
CSB-MP430675BON
CSB-EP430675BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Tryptophan synthase alpha chain (trpA) CSB-YP430676MNY
CSB-EP430676MNY
CSB-BP430676MNY
CSB-MP430676MNY
CSB-EP430676MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Probable cytosol aminopeptidase (pepA) CSB-YP430677MNY
CSB-EP430677MNY
CSB-BP430677MNY
CSB-MP430677MNY
CSB-EP430677MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 50S ribosomal protein L27 (rpmA) CSB-YP430678MNY
CSB-EP430678MNY
CSB-BP430678MNY
CSB-MP430678MNY
CSB-EP430678MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Potassium-transporting ATPase A chain (kdpA), partial CSB-YP430679MNY
CSB-EP430679MNY
CSB-BP430679MNY
CSB-MP430679MNY
CSB-EP430679MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens UPF0102 protein Mext_0406 (Mext_0406) CSB-YP430680MNY
CSB-EP430680MNY
CSB-BP430680MNY
CSB-MP430680MNY
CSB-EP430680MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (Mext_0857) CSB-YP430681MNY
CSB-EP430681MNY
CSB-BP430681MNY
CSB-MP430681MNY
CSB-EP430681MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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