Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP430642BON
CSB-EP430642BON
CSB-BP430642BON
CSB-MP430642BON
CSB-EP430642BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UvrABC system protein C (uvrC), partial CSB-YP430643BON
CSB-EP430643BON
CSB-BP430643BON
CSB-MP430643BON
CSB-EP430643BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L20 (rplT) CSB-YP430644BON
CSB-EP430644BON
CSB-BP430644BON
CSB-MP430644BON
CSB-EP430644BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Tryptophan synthase beta chain (trpB) CSB-YP430645BON
CSB-EP430645BON
CSB-BP430645BON
CSB-MP430645BON
CSB-EP430645BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis tRNA (guanine-N (7)-)-methyltransferase CSB-YP430646BON
CSB-EP430646BON
CSB-BP430646BON
CSB-MP430646BON
CSB-EP430646BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 7-cyano-7-deazaguanine synthase (queC) CSB-YP430647BON
CSB-EP430647BON
CSB-BP430647BON
CSB-MP430647BON
CSB-EP430647BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative membrane protein insertion efficiency factor (BcerKBAB4_4627) CSB-YP430648BON
CSB-EP430648BON
CSB-BP430648BON
CSB-MP430648BON
CSB-EP430648BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis DNA replication and repair protein recF (recF) CSB-YP430649BON
CSB-EP430649BON
CSB-BP430649BON
CSB-MP430649BON
CSB-EP430649BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Dihydrodipicolinate reductase (dapB) CSB-YP430650BON
CSB-EP430650BON
CSB-BP430650BON
CSB-MP430650BON
CSB-EP430650BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Xanthine phosphoribosyltransferase (xpt) CSB-YP430651BON
CSB-EP430651BON
CSB-BP430651BON
CSB-MP430651BON
CSB-EP430651BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0349 protein BcerKBAB4_4757 (BcerKBAB4_4757) CSB-YP430652BON
CSB-EP430652BON
CSB-BP430652BON
CSB-MP430652BON
CSB-EP430652BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP430653BON
CSB-EP430653BON
CSB-BP430653BON
CSB-MP430653BON
CSB-EP430653BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP430654BON
CSB-EP430654BON
CSB-BP430654BON
CSB-MP430654BON
CSB-EP430654BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L7/L12 (rplL) CSB-YP430655BON
CSB-EP430655BON
CSB-BP430655BON
CSB-MP430655BON
CSB-EP430655BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S12 (rpsL) CSB-YP430656BON
CSB-EP430656BON
CSB-BP430656BON
CSB-MP430656BON
CSB-EP430656BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L4 (rplD) CSB-YP430657BON
CSB-EP430657BON
CSB-BP430657BON
CSB-MP430657BON
CSB-EP430657BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L14 (rplN) CSB-YP430658BON
CSB-EP430658BON
CSB-BP430658BON
CSB-MP430658BON
CSB-EP430658BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S8 (rpsH) CSB-YP430659BON
CSB-EP430659BON
CSB-BP430659BON
CSB-MP430659BON
CSB-EP430659BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L36 (rpmJ) CSB-YP430660BON
CSB-EP430660BON
CSB-BP430660BON
CSB-MP430660BON
CSB-EP430660BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 10 kDa chaperonin (groS) CSB-YP430661BON
CSB-EP430661BON
CSB-BP430661BON
CSB-MP430661BON
CSB-EP430661BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>