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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium phytofermentans Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP431062CWZ
CSB-EP431062CWZ
CSB-BP431062CWZ
CSB-MP431062CWZ
CSB-EP431062CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans UPF0735 ACT domain-containing protein Cphy_3604 (Cphy_3604) CSB-YP431063CWZ
CSB-EP431063CWZ
CSB-BP431063CWZ
CSB-MP431063CWZ
CSB-EP431063CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP431064CWZ
CSB-EP431064CWZ
CSB-BP431064CWZ
CSB-MP431064CWZ
CSB-EP431064CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 30S ribosomal protein S9 (rpsI) CSB-YP431065CWZ
CSB-EP431065CWZ
CSB-BP431065CWZ
CSB-MP431065CWZ
CSB-EP431065CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Translation initiation factor IF-1 (infA) CSB-YP431066CWZ
CSB-EP431066CWZ
CSB-BP431066CWZ
CSB-MP431066CWZ
CSB-EP431066CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 50S ribosomal protein L18 (rplR) CSB-YP431067CWZ
CSB-EP431067CWZ
CSB-BP431067CWZ
CSB-MP431067CWZ
CSB-EP431067CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 50S ribosomal protein L5 (rplE) CSB-YP431068CWZ
CSB-EP431068CWZ
CSB-BP431068CWZ
CSB-MP431068CWZ
CSB-EP431068CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 50S ribosomal protein L4 (rplD) CSB-YP431069CWZ
CSB-EP431069CWZ
CSB-BP431069CWZ
CSB-MP431069CWZ
CSB-EP431069CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Urease accessory protein UreD (ureD) CSB-YP431070CWZ
CSB-EP431070CWZ
CSB-BP431070CWZ
CSB-MP431070CWZ
CSB-EP431070CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Urease subunit beta (ureB) CSB-YP431071CWZ
CSB-EP431071CWZ
CSB-BP431071CWZ
CSB-MP431071CWZ
CSB-EP431071CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans ATP synthase subunit c (atpE), partial CSB-YP431072CWZ1
CSB-EP431072CWZ1
CSB-BP431072CWZ1
CSB-MP431072CWZ1
CSB-EP431072CWZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Cell division topological specificity factor (minE) CSB-YP431073CWZ
CSB-EP431073CWZ
CSB-BP431073CWZ
CSB-MP431073CWZ
CSB-EP431073CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans DNA mismatch repair protein MutS (mutS), partial CSB-YP431074CWZ
CSB-EP431074CWZ
CSB-BP431074CWZ
CSB-MP431074CWZ
CSB-EP431074CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Ribonuclease H (rnhA) CSB-YP431075CWZ
CSB-EP431075CWZ
CSB-BP431075CWZ
CSB-MP431075CWZ
CSB-EP431075CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Ribosome maturation factor RimM (rimM) CSB-YP431076CWZ
CSB-EP431076CWZ
CSB-BP431076CWZ
CSB-MP431076CWZ
CSB-EP431076CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP431077CWZ
CSB-EP431077CWZ
CSB-BP431077CWZ
CSB-MP431077CWZ
CSB-EP431077CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Protein MraZ (mraZ) CSB-YP431078CWZ
CSB-EP431078CWZ
CSB-BP431078CWZ
CSB-MP431078CWZ
CSB-EP431078CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Elongation factor P (efp) CSB-YP431079CWZ
CSB-EP431079CWZ
CSB-BP431079CWZ
CSB-MP431079CWZ
CSB-EP431079CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Tyrosine--tRNA ligase (tyrS) CSB-YP431080CWZ
CSB-EP431080CWZ
CSB-BP431080CWZ
CSB-MP431080CWZ
CSB-EP431080CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 30S ribosomal protein S21 (rpsU) CSB-YP431081CWZ
CSB-EP431081CWZ
CSB-BP431081CWZ
CSB-MP431081CWZ
CSB-EP431081CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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