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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis bv. Antiqua UPF0319 protein YpAngola_A3206 (YpAngola_A3206) CSB-YP431462YAM
CSB-EP431462YAM
CSB-BP431462YAM
CSB-MP431462YAM
CSB-EP431462YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua DNA mismatch repair protein mutH (mutH) CSB-YP431463YAM
CSB-EP431463YAM
CSB-BP431463YAM
CSB-MP431463YAM
CSB-EP431463YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Sigma factor-binding protein crl (crl) CSB-YP431464YAM
CSB-EP431464YAM
CSB-BP431464YAM
CSB-MP431464YAM
CSB-EP431464YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP431465YAM
CSB-EP431465YAM
CSB-BP431465YAM
CSB-MP431465YAM
CSB-EP431465YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0325 protein YpAngola_A3439 (YpAngola_A3439) CSB-YP431466YAM
CSB-EP431466YAM
CSB-BP431466YAM
CSB-MP431466YAM
CSB-EP431466YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP431467YAM
CSB-EP431467YAM
CSB-BP431467YAM
CSB-MP431467YAM
CSB-EP431467YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Urease subunit alpha (ureC), partial CSB-YP431468YAM
CSB-EP431468YAM
CSB-BP431468YAM
CSB-MP431468YAM
CSB-EP431468YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein TusB (tusB) CSB-YP431469YAM
CSB-EP431469YAM
CSB-BP431469YAM
CSB-MP431469YAM
CSB-EP431469YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0270 protein YpAngola_A3698 (YpAngola_A3698) CSB-YP431470YAM
CSB-EP431470YAM
CSB-BP431470YAM
CSB-MP431470YAM
CSB-EP431470YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP431471YAM
CSB-EP431471YAM
CSB-BP431471YAM
CSB-MP431471YAM
CSB-EP431471YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Multidrug transporter MdfA (mdfA), partial CSB-YP431472YAM
CSB-EP431472YAM
CSB-BP431472YAM
CSB-MP431472YAM
CSB-EP431472YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP431473YAM
CSB-EP431473YAM
CSB-BP431473YAM
CSB-MP431473YAM
CSB-EP431473YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Met repressor (metJ) CSB-YP431474YAM
CSB-EP431474YAM
CSB-BP431474YAM
CSB-MP431474YAM
CSB-EP431474YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP431475YAM
CSB-EP431475YAM
CSB-BP431475YAM
CSB-MP431475YAM
CSB-EP431475YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP431476YAM
CSB-EP431476YAM
CSB-BP431476YAM
CSB-MP431476YAM
CSB-EP431476YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L27 (rpmA) CSB-YP431477YAM
CSB-EP431477YAM
CSB-BP431477YAM
CSB-MP431477YAM
CSB-EP431477YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP431478YAM
CSB-EP431478YAM
CSB-BP431478YAM
CSB-MP431478YAM
CSB-EP431478YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP431479YAM
CSB-EP431479YAM
CSB-BP431479YAM
CSB-MP431479YAM
CSB-EP431479YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0761 membrane protein YpAngola_A0034 (YpAngola_A0034), partial CSB-YP431480YAM1
CSB-EP431480YAM1
CSB-BP431480YAM1
CSB-MP431480YAM1
CSB-EP431480YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Orotate phosphoribosyltransferase (pyrE) CSB-YP431481YAM
CSB-EP431481YAM
CSB-BP431481YAM
CSB-MP431481YAM
CSB-EP431481YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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