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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis 50S ribosomal protein L34e (rpl34e) CSB-YP430762MNQ
CSB-EP430762MNQ
CSB-BP430762MNQ
CSB-MP430762MNQ
CSB-EP430762MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 7-cyano-7-deazaguanine tRNA-ribosyltransferase (tgtA), partial CSB-YP430763MNQ
CSB-EP430763MNQ
CSB-BP430763MNQ
CSB-MP430763MNQ
CSB-EP430763MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP430764MNQ
CSB-EP430764MNQ
CSB-BP430764MNQ
CSB-MP430764MNQ
CSB-EP430764MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Shikimate kinase (aroK) CSB-YP430765MNQ
CSB-EP430765MNQ
CSB-BP430765MNQ
CSB-MP430765MNQ
CSB-EP430765MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Proline--tRNA ligase (proS) CSB-YP430766MNQ
CSB-EP430766MNQ
CSB-BP430766MNQ
CSB-MP430766MNQ
CSB-EP430766MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0305 protein MmarC6_0221 (MmarC6_0221) CSB-YP430767MNQ
CSB-EP430767MNQ
CSB-BP430767MNQ
CSB-MP430767MNQ
CSB-EP430767MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 2 subunit beta (eIF2B) CSB-YP430768MNQ
CSB-EP430768MNQ
CSB-BP430768MNQ
CSB-MP430768MNQ
CSB-EP430768MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA-directed RNA polymerase subunit L (rpoL) CSB-YP430769MNQ
CSB-EP430769MNQ
CSB-BP430769MNQ
CSB-MP430769MNQ
CSB-EP430769MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis tRNA (guanine (26)-N (2))-dimethyltransferase CSB-YP430770MNQ
CSB-EP430770MNQ
CSB-BP430770MNQ
CSB-MP430770MNQ
CSB-EP430770MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA-binding protein MmarC6_0793 (MmarC6_0793) CSB-YP430771MNQ
CSB-EP430771MNQ
CSB-BP430771MNQ
CSB-MP430771MNQ
CSB-EP430771MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis PyrE-like protein (MmarC6_0870) CSB-YP430772MNQ
CSB-EP430772MNQ
CSB-BP430772MNQ
CSB-MP430772MNQ
CSB-EP430772MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis FO synthase subunit 2 (cofH) CSB-YP430773MNQ
CSB-EP430773MNQ
CSB-BP430773MNQ
CSB-MP430773MNQ
CSB-EP430773MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 3-dehydroquinate synthase (MmarC6_0943) CSB-YP430774MNQ
CSB-EP430774MNQ
CSB-BP430774MNQ
CSB-MP430774MNQ
CSB-EP430774MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0179 protein MmarC6_0993 (MmarC6_0993) CSB-YP430775MNQ
CSB-EP430775MNQ
CSB-BP430775MNQ
CSB-MP430775MNQ
CSB-EP430775MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Histidine--tRNA ligase (hisS) CSB-YP430776MNQ
CSB-EP430776MNQ
CSB-BP430776MNQ
CSB-MP430776MNQ
CSB-EP430776MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S19P (rps19p) CSB-YP430777MNQ
CSB-EP430777MNQ
CSB-BP430777MNQ
CSB-MP430777MNQ
CSB-EP430777MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L23P (rpl23p) CSB-YP430778MNQ
CSB-EP430778MNQ
CSB-BP430778MNQ
CSB-MP430778MNQ
CSB-EP430778MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP430779MNQ
CSB-EP430779MNQ
CSB-BP430779MNQ
CSB-MP430779MNQ
CSB-EP430779MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L11P (rpl11p) CSB-YP430780MNQ
CSB-EP430780MNQ
CSB-BP430780MNQ
CSB-MP430780MNQ
CSB-EP430780MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L18P (rpl18p) CSB-YP430781MNQ
CSB-EP430781MNQ
CSB-BP430781MNQ
CSB-MP430781MNQ
CSB-EP430781MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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