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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus maripaludis 30S ribosomal protein S3P (rps3p) CSB-YP430782MNQ
CSB-EP430782MNQ
CSB-BP430782MNQ
CSB-MP430782MNQ
CSB-EP430782MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Elongation factor 1-alpha (tuf) CSB-YP430783MNQ
CSB-EP430783MNQ
CSB-BP430783MNQ
CSB-MP430783MNQ
CSB-EP430783MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S4P (rps4p) CSB-YP430784MNQ
CSB-EP430784MNQ
CSB-BP430784MNQ
CSB-MP430784MNQ
CSB-EP430784MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP430785MNQ
CSB-EP430785MNQ
CSB-BP430785MNQ
CSB-MP430785MNQ
CSB-EP430785MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobyric acid synthase (cobQ) CSB-YP430786MNQ
CSB-EP430786MNQ
CSB-BP430786MNQ
CSB-MP430786MNQ
CSB-EP430786MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Thiamine-phosphate synthase (thiE) CSB-YP430787MNQ
CSB-EP430787MNQ
CSB-BP430787MNQ
CSB-MP430787MNQ
CSB-EP430787MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase beta chain (atpB) CSB-YP430788MNQ
CSB-EP430788MNQ
CSB-BP430788MNQ
CSB-MP430788MNQ
CSB-EP430788MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Anthranilate phosphoribosyltransferase (trpD) CSB-YP430789MNQ
CSB-EP430789MNQ
CSB-BP430789MNQ
CSB-MP430789MNQ
CSB-EP430789MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis ATP phosphoribosyltransferase (hisG) CSB-YP430790MNQ
CSB-EP430790MNQ
CSB-BP430790MNQ
CSB-MP430790MNQ
CSB-EP430790MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease P protein component 4 (rnp4) CSB-YP430791MNQ
CSB-EP430791MNQ
CSB-BP430791MNQ
CSB-MP430791MNQ
CSB-EP430791MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0145 protein MmarC6_1828 (MmarC6_1828) CSB-YP430792MNQ
CSB-EP430792MNQ
CSB-BP430792MNQ
CSB-MP430792MNQ
CSB-EP430792MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Multifunctional CCA protein (cca) CSB-YP430793BPS
CSB-EP430793BPS
CSB-BP430793BPS
CSB-MP430793BPS
CSB-EP430793BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP430794BPS
CSB-EP430794BPS
CSB-BP430794BPS
CSB-MP430794BPS
CSB-EP430794BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP430795BPS
CSB-EP430795BPS
CSB-BP430795BPS
CSB-MP430795BPS
CSB-EP430795BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP430796BPS
CSB-EP430796BPS
CSB-BP430796BPS
CSB-MP430796BPS
CSB-EP430796BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L7/L12 (rplL) CSB-YP430797BPS
CSB-EP430797BPS
CSB-BP430797BPS
CSB-MP430797BPS
CSB-EP430797BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S10 (rpsJ) CSB-YP430798BPS
CSB-EP430798BPS
CSB-BP430798BPS
CSB-MP430798BPS
CSB-EP430798BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L14 (rplN) CSB-YP430799BPS
CSB-EP430799BPS
CSB-BP430799BPS
CSB-MP430799BPS
CSB-EP430799BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L18 (rplR) CSB-YP430800BPS
CSB-EP430800BPS
CSB-BP430800BPS
CSB-MP430800BPS
CSB-EP430800BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S4 (rpsD) CSB-YP430801BPS
CSB-EP430801BPS
CSB-BP430801BPS
CSB-MP430801BPS
CSB-EP430801BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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