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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia multivorans Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP430822BPS
CSB-EP430822BPS
CSB-BP430822BPS
CSB-MP430822BPS
CSB-EP430822BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Methylglyoxal synthase (mgsA) CSB-YP430823BPS
CSB-EP430823BPS
CSB-BP430823BPS
CSB-MP430823BPS
CSB-EP430823BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Trigger factor (tig) CSB-YP430824BPS
CSB-EP430824BPS
CSB-BP430824BPS
CSB-MP430824BPS
CSB-EP430824BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Putative arginyl-tRNA--protein transferase (ate) CSB-YP430825BPS
CSB-EP430825BPS
CSB-BP430825BPS
CSB-MP430825BPS
CSB-EP430825BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Tryptophan synthase alpha chain (trpA) CSB-YP430826BPS
CSB-EP430826BPS
CSB-BP430826BPS
CSB-MP430826BPS
CSB-EP430826BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP430827BPS
CSB-EP430827BPS
CSB-BP430827BPS
CSB-MP430827BPS
CSB-EP430827BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Flavoprotein wrbA (wrbA) CSB-YP430828BPS
CSB-EP430828BPS
CSB-BP430828BPS
CSB-MP430828BPS
CSB-EP430828BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP430829HSK
CSB-EP430829HSK
CSB-BP430829HSK
CSB-MP430829HSK
CSB-EP430829HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus UPF0173 metal-dependent hydrolase Haur_4333 (Haur_4333) CSB-YP430830HSK
CSB-EP430830HSK
CSB-BP430830HSK
CSB-MP430830HSK
CSB-EP430830HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Asparagine--tRNA ligase (asnS) CSB-YP430831HSK
CSB-EP430831HSK
CSB-BP430831HSK
CSB-MP430831HSK
CSB-EP430831HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP430832HSK
CSB-EP430832HSK
CSB-BP430832HSK
CSB-MP430832HSK
CSB-EP430832HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Cobyric acid synthase (cobQ) CSB-YP430833HSK
CSB-EP430833HSK
CSB-BP430833HSK
CSB-MP430833HSK
CSB-EP430833HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 5'-nucleotidase surE (surE) CSB-YP430834HSK
CSB-EP430834HSK
CSB-BP430834HSK
CSB-MP430834HSK
CSB-EP430834HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP430835HSK
CSB-EP430835HSK
CSB-BP430835HSK
CSB-MP430835HSK
CSB-EP430835HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L23 (rplW) CSB-YP430836HSK
CSB-EP430836HSK
CSB-BP430836HSK
CSB-MP430836HSK
CSB-EP430836HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 30S ribosomal protein S14 type Z (rpsZ) CSB-YP430837HSK
CSB-EP430837HSK
CSB-BP430837HSK
CSB-MP430837HSK
CSB-EP430837HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP430838HSK
CSB-EP430838HSK
CSB-BP430838HSK
CSB-MP430838HSK
CSB-EP430838HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP430839HSK
CSB-EP430839HSK
CSB-BP430839HSK
CSB-MP430839HSK
CSB-EP430839HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus NADH-quinone oxidoreductase subunit D 1 (nuoD1) CSB-YP430840HSK
CSB-EP430840HSK
CSB-BP430840HSK
CSB-MP430840HSK
CSB-EP430840HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L11 (rplK) CSB-YP430841HSK
CSB-EP430841HSK
CSB-BP430841HSK
CSB-MP430841HSK
CSB-EP430841HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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