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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua ATP synthase gamma chain (atpG) CSB-YP432363YAM
CSB-EP432363YAM
CSB-BP432363YAM
CSB-MP432363YAM
CSB-EP432363YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP432364YAM
CSB-EP432364YAM
CSB-BP432364YAM
CSB-MP432364YAM
CSB-EP432364YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua D-tyrosyl-tRNA (Tyr) deacylase CSB-YP432365YAM
CSB-EP432365YAM
CSB-BP432365YAM
CSB-MP432365YAM
CSB-EP432365YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua DNA protection during starvation protein (dps) CSB-YP432366YAM
CSB-EP432366YAM
CSB-BP432366YAM
CSB-MP432366YAM
CSB-EP432366YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0304 protein YpAngola_A1823 (YpAngola_A1823) CSB-YP432367YAM
CSB-EP432367YAM
CSB-BP432367YAM
CSB-MP432367YAM
CSB-EP432367YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Acetate kinase (ackA) CSB-YP432368YAM
CSB-EP432368YAM
CSB-BP432368YAM
CSB-MP432368YAM
CSB-EP432368YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable rRNA maturation factor (YpAngola_A1832) CSB-YP432369YAM
CSB-EP432369YAM
CSB-BP432369YAM
CSB-MP432369YAM
CSB-EP432369YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP432370YAM
CSB-EP432370YAM
CSB-BP432370YAM
CSB-MP432370YAM
CSB-EP432370YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Bifunctional protein HldE (hldE) CSB-YP432371YAM
CSB-EP432371YAM
CSB-BP432371YAM
CSB-MP432371YAM
CSB-EP432371YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0434 protein YpAngola_A1964 (YpAngola_A1964) CSB-YP432372YAM
CSB-EP432372YAM
CSB-BP432372YAM
CSB-MP432372YAM
CSB-EP432372YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Chorismate synthase (aroC) CSB-YP432373YAM
CSB-EP432373YAM
CSB-BP432373YAM
CSB-MP432373YAM
CSB-EP432373YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Histidine--tRNA ligase (hisS) CSB-YP432374YAM
CSB-EP432374YAM
CSB-BP432374YAM
CSB-MP432374YAM
CSB-EP432374YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP432375YAM
CSB-EP432375YAM
CSB-BP432375YAM
CSB-MP432375YAM
CSB-EP432375YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0312 protein YpAngola_A2224 (YpAngola_A2224) CSB-YP432376YAM
CSB-EP432376YAM
CSB-BP432376YAM
CSB-MP432376YAM
CSB-EP432376YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L4 (rplD) CSB-YP432377YAM
CSB-EP432377YAM
CSB-BP432377YAM
CSB-MP432377YAM
CSB-EP432377YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L5 (rplE) CSB-YP432378YAM
CSB-EP432378YAM
CSB-BP432378YAM
CSB-MP432378YAM
CSB-EP432378YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Tryptophan synthase alpha chain (trpA) CSB-YP432379YAM
CSB-EP432379YAM
CSB-BP432379YAM
CSB-MP432379YAM
CSB-EP432379YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Fatty acid metabolism regulator protein (fadR) CSB-YP432380YAM
CSB-EP432380YAM
CSB-BP432380YAM
CSB-MP432380YAM
CSB-EP432380YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Probable intron-encoded endonuclease aI2 (aI2), partial CSB-YP432381DIS
CSB-EP432381DIS
CSB-BP432381DIS
CSB-MP432381DIS
CSB-EP432381DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 (PHYPADRAFT_128349) CSB-YP432382EVD
CSB-EP432382EVD
CSB-BP432382EVD
CSB-MP432382EVD
CSB-EP432382EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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