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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chicken G1/S-specific cyclin-D2 (CCND2) CSB-YP004812CH
CSB-EP004812CH
CSB-BP004812CH
CSB-MP004812CH
CSB-EP004812CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Proteasome subunit alpha type-2 (Psma2) CSB-YP018866MO
CSB-EP018866MO
CSB-BP018866MO
CSB-MP018866MO
CSB-EP018866MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Retinoic acid receptor RXR-beta (Rxrb) CSB-YP020613RA
CSB-EP020613RA
CSB-BP020613RA
CSB-MP020613RA
CSB-EP020613RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human YLP motif-containing protein 1 (YLPM1), partial CSB-YP026266HU
CSB-EP026266HU
CSB-BP026266HU
CSB-MP026266HU
CSB-EP026266HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Dual specificity protein kinase CLK2 (CLK2), partial CSB-YP005558HU1
CSB-BP005558HU1
CSB-MP005558HU1
CSB-EP005558HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Presenilin-1 (PSEN1), partial CSB-YP018846HU
CSB-EP018846HU
CSB-BP018846HU
CSB-MP018846HU
CSB-EP018846HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Retinoic acid receptor responder protein 1 (RARRES1), partial CSB-YP019341HU1
CSB-EP019341HU1
CSB-BP019341HU1
CSB-MP019341HU1
CSB-EP019341HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Regulator of G-protein signaling 19 (RGS19) CSB-YP019650HU
CSB-EP019650HU
CSB-BP019650HU
CSB-MP019650HU
CSB-EP019650HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Myogenin (MYOG) CSB-YP015360PI
CSB-EP015360PI
CSB-BP015360PI
CSB-MP015360PI
CSB-EP015360PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog NADH dehydrogenase [ubiquinone] flavoprotein 2 (NDUFV2) CSB-YP015669DO
CSB-EP015669DO
CSB-BP015669DO
CSB-MP015669DO
CSB-EP015669DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein kinase 19 (STK19) CSB-YP022839HU
CSB-EP022839HU
CSB-BP022839HU
CSB-MP022839HU
CSB-EP022839HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta (Rhesus macaque) Estrogen receptor (ESR1) CSB-YP007830MOW
CSB-EP007830MOW
CSB-BP007830MOW
CSB-MP007830MOW
CSB-EP007830MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA ligase 4 (LIG4), partial CSB-YP012932HU
CSB-EP012932HU
CSB-BP012932HU
CSB-MP012932HU
CSB-EP012932HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Homeobox protein Hox-C6 (HOXC6) CSB-YP010677SH
CSB-EP010677SH
CSB-BP010677SH
CSB-MP010677SH
CSB-EP010677SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Retinol-binding protein 2 (RBP2) CSB-YP019481HU
CSB-BP019481HU
CSB-MP019481HU
CSB-EP019481HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta (Rhesus macaque) 5-hydroxytryptamine receptor 2A (HTR2A), partial CSB-YP010887MOW1
CSB-EP010887MOW1
CSB-BP010887MOW1
CSB-MP010887MOW1
CSB-EP010887MOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histamine N-methyltransferase (HNMT) CSB-YP010598HU
CSB-EP010598HU
CSB-BP010598HU
CSB-MP010598HU
CSB-EP010598HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Guanine nucleotide-binding protein G (i) subunit alpha-2 (GNAI2) CSB-YP009589CH
CSB-EP009589CH
CSB-BP009589CH
CSB-MP009589CH
CSB-EP009589CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Insulin gene enhancer protein ISL-1 (ISL1) CSB-YP011846CH
CSB-EP011846CH
CSB-BP011846CH
CSB-MP011846CH
CSB-EP011846CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sulfotransferase 1A2 (SULT1A2) CSB-YP022934HU
CSB-EP022934HU
CSB-BP022934HU
CSB-MP022934HU
CSB-EP022934HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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