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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Stathmin (Stmn1) CSB-YP022857MO
CSB-EP022857MO
CSB-BP022857MO
CSB-MP022857MO
CSB-EP022857MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Huntingtin-associated protein 1 (HAP1) CSB-YP010129HU
CSB-EP010129HU
CSB-BP010129HU
CSB-MP010129HU
CSB-EP010129HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Telomeric repeat-binding factor 1 (TERF1) CSB-YP023386HU
CSB-EP023386HU
CSB-BP023386HU
CSB-MP023386HU
CSB-EP023386HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 14 (USP14) CSB-YP025704HU
CSB-BP025704HU
CSB-MP025704HU
CSB-EP025704HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Potassium-transporting ATPase alpha chain 2 (ATP12A), partial CSB-YP002316HU
CSB-EP002316HU
CSB-BP002316HU
CSB-MP002316HU
CSB-EP002316HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human UV excision repair protein RAD23 homolog B (RAD23B) CSB-YP019260HU
CSB-EP019260HU
CSB-BP019260HU
CSB-MP019260HU
CSB-EP019260HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (St3gal1), partial CSB-YP022751MO1
CSB-EP022751MO1
CSB-BP022751MO1
CSB-MP022751MO1
CSB-EP022751MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Ephrin type-A receptor 5 (EPHA5), partial CSB-YP007725CH
CSB-EP007725CH
CSB-BP007725CH
CSB-MP007725CH
CSB-EP007725CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ephrin type-A receptor 6 (Epha6), partial CSB-YP007726RA
CSB-EP007726RA
CSB-BP007726RA
CSB-MP007726RA
CSB-EP007726RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ephrin type-A receptor 4 (EPHA4), partial CSB-YP007724HU
CSB-EP007724HU
CSB-BP007724HU
CSB-MP007724HU
CSB-EP007724HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 26S protease regulatory subunit 6B (Psmc4) CSB-YP018894MO
CSB-EP018894MO
CSB-BP018894MO
CSB-MP018894MO
CSB-EP018894MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Alpha-N-acetylglucosaminidase (NAGLU) CSB-YP015416HU
CSB-EP015416HU
CSB-BP015416HU
CSB-MP015416HU
CSB-EP015416HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tyrosine-protein phosphatase non-receptor type 5 (PTPN5), partial CSB-YP019042HU
CSB-BP019042HU
CSB-MP019042HU
CSB-EP019042HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transcription factor Maf (Maf) CSB-YP013312MO
CSB-EP013312MO
CSB-BP013312MO
CSB-MP013312MO
CSB-EP013312MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Epithelial membrane protein 3 (EMP3), partial CSB-YP007650HU1
CSB-EP007650HU1
CSB-BP007650HU1
CSB-MP007650HU1
CSB-EP007650HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Hydroxymethylglutaryl-CoA synthase, mitochondrial (HMGCS2) CSB-YP010567HU
CSB-EP010567HU
CSB-BP010567HU
CSB-MP010567HU
CSB-EP010567HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Microfibrillar-associated protein 2 (MFAP2) CSB-YP013746HU
CSB-EP013746HU
CSB-BP013746HU
CSB-MP013746HU
CSB-EP013746HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Solute carrier family 12 member 2 (Slc12a2), partial CSB-YP021383MO
CSB-EP021383MO
CSB-BP021383MO
CSB-MP021383MO
CSB-EP021383MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat GTP-binding protein RAD (Rrad) CSB-YP020507RA
CSB-EP020507RA
CSB-BP020507RA
CSB-MP020507RA
CSB-EP020507RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Bax inhibitor 1 (TMBIM6), partial CSB-YP023632HU1
CSB-EP023632HU1
CSB-BP023632HU1
CSB-MP023632HU1
CSB-EP023632HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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