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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter lovleyi NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP451316GFO1
CSB-EP451316GFO1
CSB-BP451316GFO1
CSB-MP451316GFO1
CSB-EP451316GFO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi ATP synthase epsilon chain (atpC) CSB-YP451317GFO
CSB-EP451317GFO
CSB-BP451317GFO
CSB-MP451317GFO
CSB-EP451317GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi UPF0173 metal-dependent hydrolase Glov_0171 (Glov_0171) CSB-YP451318GFO
CSB-EP451318GFO
CSB-BP451318GFO
CSB-MP451318GFO
CSB-EP451318GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Potassium-transporting ATPase C chain (kdpC), partial CSB-YP451319GFO
CSB-EP451319GFO
CSB-BP451319GFO
CSB-MP451319GFO
CSB-EP451319GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Flagellar hook-basal body complex protein FliE (fliE) CSB-YP451320GFO
CSB-EP451320GFO
CSB-BP451320GFO
CSB-MP451320GFO
CSB-EP451320GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Methionine--tRNA ligase (metG), partial CSB-YP451321DSR
CSB-EP451321DSR
CSB-BP451321DSR
CSB-MP451321DSR
CSB-EP451321DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Arginine repressor (argR) CSB-YP451322DSR
CSB-EP451322DSR
CSB-BP451322DSR
CSB-MP451322DSR
CSB-EP451322DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Protease HtpX homolog (htpX), partial CSB-YP451323DSR1
CSB-EP451323DSR1
CSB-BP451323DSR1
CSB-MP451323DSR1
CSB-EP451323DSR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola D-alanine--D-alanine ligase (ddl) CSB-YP451324DSR
CSB-EP451324DSR
CSB-BP451324DSR
CSB-MP451324DSR
CSB-EP451324DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Proline--tRNA ligase (proS) CSB-YP451325DSR
CSB-EP451325DSR
CSB-BP451325DSR
CSB-MP451325DSR
CSB-EP451325DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S6 (rpsF) CSB-YP451326DSR
CSB-EP451326DSR
CSB-BP451326DSR
CSB-MP451326DSR
CSB-EP451326DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L32 (rpmF) CSB-YP451327DSR
CSB-EP451327DSR
CSB-BP451327DSR
CSB-MP451327DSR
CSB-EP451327DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Probable endonuclease 4 (nfo) CSB-YP451328DSR
CSB-EP451328DSR
CSB-BP451328DSR
CSB-MP451328DSR
CSB-EP451328DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP451329DSR
CSB-EP451329DSR
CSB-BP451329DSR
CSB-MP451329DSR
CSB-EP451329DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S15 (rpsO) CSB-YP451330DSR
CSB-EP451330DSR
CSB-BP451330DSR
CSB-MP451330DSR
CSB-EP451330DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola DNA ligase (ligA), partial CSB-YP451331DSR
CSB-EP451331DSR
CSB-BP451331DSR
CSB-MP451331DSR
CSB-EP451331DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Indole-3-glycerol phosphate synthase (trpC) CSB-YP451332DSR
CSB-EP451332DSR
CSB-BP451332DSR
CSB-MP451332DSR
CSB-EP451332DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 10 kDa chaperonin (groS) CSB-YP451333DSR
CSB-EP451333DSR
CSB-BP451333DSR
CSB-MP451333DSR
CSB-EP451333DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP451334DSR
CSB-EP451334DSR
CSB-BP451334DSR
CSB-MP451334DSR
CSB-EP451334DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Glycogen synthase (glgA) CSB-YP451335DSR
CSB-EP451335DSR
CSB-BP451335DSR
CSB-MP451335DSR
CSB-EP451335DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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