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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Wolbachia pipientis subsp. Culex pipiens Aspartate--tRNA ligase (aspS), partial CSB-YP451236WBO
CSB-EP451236WBO
CSB-BP451236WBO
CSB-MP451236WBO
CSB-EP451236WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Elongation factor 4 (lepA), partial CSB-YP451237WBO
CSB-EP451237WBO
CSB-BP451237WBO
CSB-MP451237WBO
CSB-EP451237WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 10 kDa chaperonin (groS) CSB-YP451238OEV
CSB-EP451238OEV
CSB-BP451238OEV
CSB-MP451238OEV
CSB-EP451238OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi SsrA-binding protein (smpB) CSB-YP451239OEV
CSB-EP451239OEV
CSB-BP451239OEV
CSB-MP451239OEV
CSB-EP451239OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Serine--tRNA ligase (serS) CSB-YP451240OEV
CSB-EP451240OEV
CSB-BP451240OEV
CSB-MP451240OEV
CSB-EP451240OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP451241OEV
CSB-EP451241OEV
CSB-BP451241OEV
CSB-MP451241OEV
CSB-EP451241OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L35 (rpmI) CSB-YP451242OEV
CSB-EP451242OEV
CSB-BP451242OEV
CSB-MP451242OEV
CSB-EP451242OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP451243OEV
CSB-EP451243OEV
CSB-BP451243OEV
CSB-MP451243OEV
CSB-EP451243OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP451244OEV
CSB-EP451244OEV
CSB-BP451244OEV
CSB-MP451244OEV
CSB-EP451244OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L16 (rplP) CSB-YP451245OEV
CSB-EP451245OEV
CSB-BP451245OEV
CSB-MP451245OEV
CSB-EP451245OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S8 (rpsH) CSB-YP451246OEV
CSB-EP451246OEV
CSB-BP451246OEV
CSB-MP451246OEV
CSB-EP451246OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Adenylate kinase (adk) CSB-YP451247OEV
CSB-EP451247OEV
CSB-BP451247OEV
CSB-MP451247OEV
CSB-EP451247OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S20 (rpsT) CSB-YP451248OEV
CSB-EP451248OEV
CSB-BP451248OEV
CSB-MP451248OEV
CSB-EP451248OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S21 (rpsU) CSB-YP451249OEV
CSB-EP451249OEV
CSB-BP451249OEV
CSB-MP451249OEV
CSB-EP451249OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Putative Holliday junction resolvase (OTT_1426) CSB-YP451250OEV
CSB-EP451250OEV
CSB-BP451250OEV
CSB-MP451250OEV
CSB-EP451250OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP451251OEV
CSB-EP451251OEV
CSB-BP451251OEV
CSB-MP451251OEV
CSB-EP451251OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L28 (rpmB) CSB-YP451252OEV
CSB-EP451252OEV
CSB-BP451252OEV
CSB-MP451252OEV
CSB-EP451252OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UNC119-binding protein C5orf30 homolog (zgc:194273, zgc:194281) CSB-YP451253DIL
CSB-EP451253DIL
CSB-BP451253DIL
CSB-MP451253DIL
CSB-EP451253DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Chaperone protein DnaK (dnaK), partial CSB-YP451254BTA
CSB-EP451254BTA
CSB-BP451254BTA
CSB-MP451254BTA
CSB-EP451254BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP451255BTA
CSB-EP451255BTA
CSB-BP451255BTA
CSB-MP451255BTA
CSB-EP451255BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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