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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus fermentum Transcription elongation factor GreA (greA) CSB-YP452317LMO
CSB-EP452317LMO
CSB-BP452317LMO
CSB-MP452317LMO
CSB-EP452317LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum S-adenosylmethionine synthase (metK) CSB-YP452318LMO
CSB-EP452318LMO
CSB-BP452318LMO
CSB-MP452318LMO
CSB-EP452318LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L17 (rplQ) CSB-YP452319LMO
CSB-EP452319LMO
CSB-BP452319LMO
CSB-MP452319LMO
CSB-EP452319LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L30 (rpmD) CSB-YP452320LMO
CSB-EP452320LMO
CSB-BP452320LMO
CSB-MP452320LMO
CSB-EP452320LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L14 (rplN) CSB-YP452321LMO
CSB-EP452321LMO
CSB-BP452321LMO
CSB-MP452321LMO
CSB-EP452321LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L2 (rplB) CSB-YP452322LMO
CSB-EP452322LMO
CSB-BP452322LMO
CSB-MP452322LMO
CSB-EP452322LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Aspartate--ammonia ligase (asnA) CSB-YP452323LMO
CSB-EP452323LMO
CSB-BP452323LMO
CSB-MP452323LMO
CSB-EP452323LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum D-ribose pyranase (rbsD) CSB-YP452324LMO
CSB-EP452324LMO
CSB-BP452324LMO
CSB-MP452324LMO
CSB-EP452324LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Chromosomal replication initiator protein DnaA (dnaA) CSB-YP452325LMO
CSB-EP452325LMO
CSB-BP452325LMO
CSB-MP452325LMO
CSB-EP452325LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP452326LMO
CSB-EP452326LMO
CSB-BP452326LMO
CSB-MP452326LMO
CSB-EP452326LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Ketol-acid reductoisomerase (ilvC) CSB-YP452327KBO
CSB-EP452327KBO
CSB-BP452327KBO
CSB-MP452327KBO
CSB-EP452327KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Cysteine--tRNA ligase (cysS) CSB-YP452328KBO
CSB-EP452328KBO
CSB-BP452328KBO
CSB-MP452328KBO
CSB-EP452328KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP452329KBO
CSB-EP452329KBO
CSB-BP452329KBO
CSB-MP452329KBO
CSB-EP452329KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila UvrABC system protein C (uvrC), partial CSB-YP452330KBO
CSB-EP452330KBO
CSB-BP452330KBO
CSB-MP452330KBO
CSB-EP452330KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila UPF0317 protein KRH_21160 (KRH_21160) CSB-YP452331KBO
CSB-EP452331KBO
CSB-BP452331KBO
CSB-MP452331KBO
CSB-EP452331KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 30S ribosomal protein S20 (rpsT) CSB-YP452332KBO
CSB-EP452332KBO
CSB-BP452332KBO
CSB-MP452332KBO
CSB-EP452332KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Urease subunit gamma (ureA) CSB-YP452333KBO
CSB-EP452333KBO
CSB-BP452333KBO
CSB-MP452333KBO
CSB-EP452333KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila D-tyrosyl-tRNA (Tyr) deacylase CSB-YP452334KBO
CSB-EP452334KBO
CSB-BP452334KBO
CSB-MP452334KBO
CSB-EP452334KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP452335KBO
CSB-EP452335KBO
CSB-BP452335KBO
CSB-MP452335KBO
CSB-EP452335KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila 30S ribosomal protein S3 (rpsC) CSB-YP452336KBO
CSB-EP452336KBO
CSB-BP452336KBO
CSB-MP452336KBO
CSB-EP452336KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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