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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 Nitric oxide reductase FlRd-NAD (+) reductase CSB-YP452917SYZ
CSB-EP452917SYZ
CSB-BP452917SYZ
CSB-MP452917SYZ
CSB-EP452917SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0597 protein yhaM (yhaM) CSB-YP452918SYZ
CSB-EP452918SYZ
CSB-BP452918SYZ
CSB-MP452918SYZ
CSB-EP452918SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 tRNA-modifying protein ygfZ (ygfZ) CSB-YP452919SYZ
CSB-EP452919SYZ
CSB-BP452919SYZ
CSB-MP452919SYZ
CSB-EP452919SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cell division protein ZapA (zapA) CSB-YP452920SYZ
CSB-EP452920SYZ
CSB-BP452920SYZ
CSB-MP452920SYZ
CSB-EP452920SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0301 protein yqgE (yqgE) CSB-YP452921SYZ
CSB-EP452921SYZ
CSB-BP452921SYZ
CSB-MP452921SYZ
CSB-EP452921SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP452922SYZ
CSB-EP452922SYZ
CSB-BP452922SYZ
CSB-MP452922SYZ
CSB-EP452922SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Undecaprenyl-diphosphatase (uppP), partial CSB-YP452923SYZ1
CSB-EP452923SYZ1
CSB-BP452923SYZ1
CSB-MP452923SYZ1
CSB-EP452923SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 DNA replication terminus site-binding protein (tus) CSB-YP452924SYZ
CSB-EP452924SYZ
CSB-BP452924SYZ
CSB-MP452924SYZ
CSB-EP452924SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 30S ribosomal protein S9 (rpsI) CSB-YP452925SYZ
CSB-EP452925SYZ
CSB-BP452925SYZ
CSB-MP452925SYZ
CSB-EP452925SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L27 (rpmA) CSB-YP452926SYZ
CSB-EP452926SYZ
CSB-BP452926SYZ
CSB-MP452926SYZ
CSB-EP452926SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosome maturation factor RimP (rimP) CSB-YP452927SYZ
CSB-EP452927SYZ
CSB-BP452927SYZ
CSB-MP452927SYZ
CSB-EP452927SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 30S ribosomal protein S20 (rpsT) CSB-YP452928SYZ
CSB-EP452928SYZ
CSB-BP452928SYZ
CSB-MP452928SYZ
CSB-EP452928SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP452929SYZ
CSB-EP452929SYZ
CSB-BP452929SYZ
CSB-MP452929SYZ
CSB-EP452929SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP452930SYZ
CSB-EP452930SYZ
CSB-BP452930SYZ
CSB-MP452930SYZ
CSB-EP452930SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein translocase subunit SecA (secA), partial CSB-YP452931SYZ
CSB-EP452931SYZ
CSB-BP452931SYZ
CSB-MP452931SYZ
CSB-EP452931SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP452932SYZ
CSB-EP452932SYZ
CSB-BP452932SYZ
CSB-MP452932SYZ
CSB-EP452932SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Putative phosphotransferase ydiA (ydiA) CSB-YP452933SYZ
CSB-EP452933SYZ
CSB-BP452933SYZ
CSB-MP452933SYZ
CSB-EP452933SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Shikimate dehydrogenase (aroE) CSB-YP452934SYZ
CSB-EP452934SYZ
CSB-BP452934SYZ
CSB-MP452934SYZ
CSB-EP452934SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 30S ribosomal protein S4 (rpsD) CSB-YP452935SYZ
CSB-EP452935SYZ
CSB-BP452935SYZ
CSB-MP452935SYZ
CSB-EP452935SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L6 (rplF) CSB-YP452936SYZ
CSB-EP452936SYZ
CSB-BP452936SYZ
CSB-MP452936SYZ
CSB-EP452936SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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