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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP451556RLV
CSB-EP451556RLV
CSB-BP451556RLV
CSB-MP451556RLV
CSB-EP451556RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP451557RLV1
CSB-EP451557RLV1
CSB-BP451557RLV1
CSB-MP451557RLV1
CSB-EP451557RLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris GTPase obg (obg) CSB-YP451558RLV
CSB-EP451558RLV
CSB-BP451558RLV
CSB-MP451558RLV
CSB-EP451558RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S4 (rpsD) CSB-YP451559RLV
CSB-EP451559RLV
CSB-BP451559RLV
CSB-MP451559RLV
CSB-EP451559RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S2 (rpsB) CSB-YP451560RLV
CSB-EP451560RLV
CSB-BP451560RLV
CSB-MP451560RLV
CSB-EP451560RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Lipoprotein signal peptidase (lspA), partial CSB-YP451561RLV1
CSB-EP451561RLV1
CSB-BP451561RLV1
CSB-MP451561RLV1
CSB-EP451561RLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0434 protein Rpal_0270 (Rpal_0270) CSB-YP451562RLV
CSB-EP451562RLV
CSB-BP451562RLV
CSB-MP451562RLV
CSB-EP451562RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Putative phosphotransferase Rpal_0300 (Rpal_0300) CSB-YP451563RLV
CSB-EP451563RLV
CSB-BP451563RLV
CSB-MP451563RLV
CSB-EP451563RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Histidinol-phosphate aminotransferase (hisC) CSB-YP451564RLV
CSB-EP451564RLV
CSB-BP451564RLV
CSB-MP451564RLV
CSB-EP451564RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Chaperone protein DnaK (dnaK), partial CSB-YP451565RLV
CSB-EP451565RLV
CSB-BP451565RLV
CSB-MP451565RLV
CSB-EP451565RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP451566RLV
CSB-EP451566RLV
CSB-BP451566RLV
CSB-MP451566RLV
CSB-EP451566RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Aspartate--tRNA ligase (aspS), partial CSB-YP451567RLV
CSB-EP451567RLV
CSB-BP451567RLV
CSB-MP451567RLV
CSB-EP451567RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP451568RLV
CSB-EP451568RLV
CSB-BP451568RLV
CSB-MP451568RLV
CSB-EP451568RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP451569RLV
CSB-EP451569RLV
CSB-BP451569RLV
CSB-MP451569RLV
CSB-EP451569RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0235 protein Rpal_0418 (Rpal_0418) CSB-YP451570RLV
CSB-EP451570RLV
CSB-BP451570RLV
CSB-MP451570RLV
CSB-EP451570RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosome maturation factor RimP (rimP) CSB-YP451571RLV
CSB-EP451571RLV
CSB-BP451571RLV
CSB-MP451571RLV
CSB-EP451571RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP451572RLV
CSB-EP451572RLV
CSB-BP451572RLV
CSB-MP451572RLV
CSB-EP451572RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP451573RLV
CSB-EP451573RLV
CSB-BP451573RLV
CSB-MP451573RLV
CSB-EP451573RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S11 (rpsK) CSB-YP451574RLV
CSB-EP451574RLV
CSB-BP451574RLV
CSB-MP451574RLV
CSB-EP451574RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L6 (rplF) CSB-YP451575RLV
CSB-EP451575RLV
CSB-BP451575RLV
CSB-MP451575RLV
CSB-EP451575RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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