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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oedogonium cardiacum 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP453157ODX
CSB-EP453157ODX
CSB-BP453157ODX
CSB-MP453157ODX
CSB-EP453157ODX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP453158HWH1
CSB-EP453158HWH1
CSB-BP453158HWH1
CSB-MP453158HWH1
CSB-EP453158HWH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP453159HWH
CSB-EP453159HWH
CSB-BP453159HWH
CSB-MP453159HWH
CSB-EP453159HWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP453160HWG
CSB-EP453160HWG
CSB-BP453160HWG
CSB-MP453160HWG
CSB-EP453160HWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo 50S ribosomal protein L5, chloroplastic (rpl5) CSB-YP453161HWG
CSB-EP453161HWG
CSB-BP453161HWG
CSB-MP453161HWG
CSB-EP453161HWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP453162FEC
CSB-EP453162FEC
CSB-BP453162FEC
CSB-MP453162FEC
CSB-EP453162FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP453163FEC
CSB-EP453163FEC
CSB-BP453163FEC
CSB-MP453163FEC
CSB-EP453163FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP453164FEC
CSB-EP453164FEC
CSB-BP453164FEC
CSB-MP453164FEC
CSB-EP453164FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Photosystem II reaction center protein M (psbM), partial CSB-YP453165WBL
CSB-EP453165WBL
CSB-BP453165WBL
CSB-MP453165WBL
CSB-EP453165WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis envelope membrane protein, chloroplastic (cemA), partial CSB-YP453166WBL1
CSB-EP453166WBL1
CSB-BP453166WBL1
CSB-MP453166WBL1
CSB-EP453166WBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Photosystem I reaction center subunit IX (psaJ), partial CSB-YP453167WBL
CSB-EP453167WBL
CSB-BP453167WBL
CSB-MP453167WBL
CSB-EP453167WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP453168WBL
CSB-EP453168WBL
CSB-BP453168WBL
CSB-MP453168WBL
CSB-EP453168WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP453169WBL
CSB-EP453169WBL
CSB-BP453169WBL
CSB-MP453169WBL
CSB-EP453169WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis 30S ribosomal protein S15, chloroplastic (rps15) CSB-YP453170WBL
CSB-EP453170WBL
CSB-BP453170WBL
CSB-MP453170WBL
CSB-EP453170WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Drosulfakinins (Dsk) CSB-YP453171DMR
CSB-EP453171DMR
CSB-BP453171DMR
CSB-MP453171DMR
CSB-EP453171DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crassostrea virginica Lysozyme 3 (lysoz3) CSB-YP453172DXR
CSB-EP453172DXR
CSB-BP453172DXR
CSB-MP453172DXR
CSB-EP453172DXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops atrox Venom prothrombin activator, partial CSB-YP453173BUU
CSB-EP453173BUU
CSB-BP453173BUU
CSB-MP453173BUU
CSB-EP453173BUU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Melanoplus sanguinipes Serine protease inhibitor 2 CSB-YP453174MQU
CSB-EP453174MQU
CSB-BP453174MQU
CSB-MP453174MQU
CSB-EP453174MQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea SCP domain-containing protein 2 CSB-YP453175LQP
CSB-EP453175LQP
CSB-BP453175LQP
CSB-MP453175LQP
CSB-EP453175LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Putative peptidyl-prolyl cis-trans isomerase CSB-YP453176LQP
CSB-EP453176LQP
CSB-BP453176LQP
CSB-MP453176LQP
CSB-EP453176LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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