Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L27 (rpmA) CSB-YP453357AXI
CSB-EP453357AXI
CSB-BP453357AXI
CSB-MP453357AXI
CSB-EP453357AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Lipid-A-disaccharide synthase (lpxB) CSB-YP453358AXI
CSB-EP453358AXI
CSB-BP453358AXI
CSB-MP453358AXI
CSB-EP453358AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 GTPase obg (obg) CSB-YP453359AXI
CSB-EP453359AXI
CSB-BP453359AXI
CSB-MP453359AXI
CSB-EP453359AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Sulfurtransferase TusA homolog (tusA) CSB-YP453360AXI
CSB-EP453360AXI
CSB-BP453360AXI
CSB-MP453360AXI
CSB-EP453360AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP453361AXI
CSB-EP453361AXI
CSB-BP453361AXI
CSB-MP453361AXI
CSB-EP453361AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Recombination-associated protein rdgC (rdgC) CSB-YP453362AXI
CSB-EP453362AXI
CSB-BP453362AXI
CSB-MP453362AXI
CSB-EP453362AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0301 protein APP7_0234 (APP7_0234) CSB-YP453363AXI
CSB-EP453363AXI
CSB-BP453363AXI
CSB-MP453363AXI
CSB-EP453363AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0042 nucleotide-binding protein APP7_0339 (APP7_0339) CSB-YP453364AXI
CSB-EP453364AXI
CSB-BP453364AXI
CSB-MP453364AXI
CSB-EP453364AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP453365AXI
CSB-EP453365AXI
CSB-BP453365AXI
CSB-MP453365AXI
CSB-EP453365AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP453366AXI
CSB-EP453366AXI
CSB-BP453366AXI
CSB-MP453366AXI
CSB-EP453366AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP453367AXI
CSB-EP453367AXI
CSB-BP453367AXI
CSB-MP453367AXI
CSB-EP453367AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP453368AXI
CSB-EP453368AXI
CSB-BP453368AXI
CSB-MP453368AXI
CSB-EP453368AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP453369AXI
CSB-EP453369AXI
CSB-BP453369AXI
CSB-MP453369AXI
CSB-EP453369AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP453370AXI
CSB-EP453370AXI
CSB-BP453370AXI
CSB-MP453370AXI
CSB-EP453370AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 SsrA-binding protein (smpB) CSB-YP453371AXI
CSB-EP453371AXI
CSB-BP453371AXI
CSB-MP453371AXI
CSB-EP453371AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribose-5-phosphate isomerase A (rpiA) CSB-YP453372AXI
CSB-EP453372AXI
CSB-BP453372AXI
CSB-MP453372AXI
CSB-EP453372AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Pyridoxamine kinase (pdxY) CSB-YP453373AXI
CSB-EP453373AXI
CSB-BP453373AXI
CSB-MP453373AXI
CSB-EP453373AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Large-conductance mechanosensitive channel (mscL), partial CSB-YP453374AXI1
CSB-EP453374AXI1
CSB-BP453374AXI1
CSB-MP453374AXI1
CSB-EP453374AXI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Urease subunit beta (ureB) CSB-YP453375AXI
CSB-EP453375AXI
CSB-BP453375AXI
CSB-MP453375AXI
CSB-EP453375AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ATP synthase epsilon chain (atpC) CSB-YP453376AXI
CSB-EP453376AXI
CSB-BP453376AXI
CSB-MP453376AXI
CSB-EP453376AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>