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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum 4-hydroxy-2-oxovalerate aldolase 2 (MMAR_5021) CSB-YP452357MVS
CSB-EP452357MVS
CSB-BP452357MVS
CSB-MP452357MVS
CSB-EP452357MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L9 (rplI) CSB-YP452358MVS
CSB-EP452358MVS
CSB-BP452358MVS
CSB-MP452358MVS
CSB-EP452358MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP452359MVS
CSB-EP452359MVS
CSB-BP452359MVS
CSB-MP452359MVS
CSB-EP452359MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Long-chain-fatty-acid--AMP ligase FadD26 (fadD26), partial CSB-YP452360MVS
CSB-EP452360MVS
CSB-BP452360MVS
CSB-MP452360MVS
CSB-EP452360MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S16 (rpsP) CSB-YP452361MVS
CSB-EP452361MVS
CSB-BP452361MVS
CSB-MP452361MVS
CSB-EP452361MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP452362MVS
CSB-EP452362MVS
CSB-BP452362MVS
CSB-MP452362MVS
CSB-EP452362MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Regulatory protein recX (recX) CSB-YP452363MVS
CSB-EP452363MVS
CSB-BP452363MVS
CSB-MP452363MVS
CSB-EP452363MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative phenylalanine aminotransferase (pat) CSB-YP452364MVS
CSB-EP452364MVS
CSB-BP452364MVS
CSB-MP452364MVS
CSB-EP452364MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0678 fatty acid-binding protein-like protein MMAR_1995 (MMAR_1995) CSB-YP452365MVS
CSB-EP452365MVS
CSB-BP452365MVS
CSB-MP452365MVS
CSB-EP452365MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S20 (rpsT) CSB-YP452366MVS
CSB-EP452366MVS
CSB-BP452366MVS
CSB-MP452366MVS
CSB-EP452366MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Prephenate dehydratase (pheA) CSB-YP452367MVS
CSB-EP452367MVS
CSB-BP452367MVS
CSB-MP452367MVS
CSB-EP452367MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP452368MVS
CSB-EP452368MVS
CSB-BP452368MVS
CSB-MP452368MVS
CSB-EP452368MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Shikimate kinase (aroK) CSB-YP452369MVS
CSB-EP452369MVS
CSB-BP452369MVS
CSB-MP452369MVS
CSB-EP452369MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP452370MVS
CSB-EP452370MVS
CSB-BP452370MVS
CSB-MP452370MVS
CSB-EP452370MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L34 (rpmH) CSB-YP452371MVS
CSB-EP452371MVS
CSB-BP452371MVS
CSB-MP452371MVS
CSB-EP452371MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Pyrrolidone-carboxylate peptidase (pcp) CSB-YP452372MVS
CSB-EP452372MVS
CSB-BP452372MVS
CSB-MP452372MVS
CSB-EP452372MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0353 protein MMAR_2288 (MMAR_2288), partial CSB-YP452373MVS1
CSB-EP452373MVS1
CSB-BP452373MVS1
CSB-MP452373MVS1
CSB-EP452373MVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (MMAR_2384) CSB-YP452374MVS
CSB-EP452374MVS
CSB-BP452374MVS
CSB-MP452374MVS
CSB-EP452374MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Phosphomethylpyrimidine synthase (thiC), partial CSB-YP452375MVS
CSB-EP452375MVS
CSB-BP452375MVS
CSB-MP452375MVS
CSB-EP452375MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Tryptophan synthase alpha chain (trpA) CSB-YP452376MVS
CSB-EP452376MVS
CSB-BP452376MVS
CSB-MP452376MVS
CSB-EP452376MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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