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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila virilis Ubiquitin carboxyl-terminal hydrolase calypso (calypso) CSB-YP451876DMP
CSB-EP451876DMP
CSB-BP451876DMP
CSB-MP451876DMP
CSB-EP451876DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Histone-arginine methyltransferase CARMER (Art4), partial CSB-YP451877DMP
CSB-EP451877DMP
CSB-BP451877DMP
CSB-MP451877DMP
CSB-EP451877DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis tRNA (guanine-N (7)-)-methyltransferase subunit wuho CSB-YP451878DMP
CSB-EP451878DMP
CSB-BP451878DMP
CSB-MP451878DMP
CSB-EP451878DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Eukaryotic translation initiation factor 3 subunit F-2 (eIF3-S5-2) CSB-YP451879DMP
CSB-EP451879DMP
CSB-BP451879DMP
CSB-MP451879DMP
CSB-EP451879DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Protein king tubby (king-tubby) CSB-YP451880DMQ
CSB-EP451880DMQ
CSB-BP451880DMQ
CSB-MP451880DMQ
CSB-EP451880DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Protein CLP1 homolog (cbc) CSB-YP451881DMQ
CSB-EP451881DMQ
CSB-BP451881DMQ
CSB-MP451881DMQ
CSB-EP451881DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Adenosine monophosphate-protein transferase FICD homolog (GK14760), partial CSB-YP451882DMQ1
CSB-EP451882DMQ1
CSB-BP451882DMQ1
CSB-MP451882DMQ1
CSB-EP451882DMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni SOSS complex subunit C homolog B (GK20175) CSB-YP451883DMQ
CSB-EP451883DMQ
CSB-BP451883DMQ
CSB-MP451883DMQ
CSB-EP451883DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Calcium channel flower (flower), partial CSB-YP451884DMQ1
CSB-EP451884DMQ1
CSB-BP451884DMQ1
CSB-MP451884DMQ1
CSB-EP451884DMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Elongation factor G, mitochondrial (ico), partial CSB-YP451885DMQ
CSB-EP451885DMQ
CSB-BP451885DMQ
CSB-MP451885DMQ
CSB-EP451885DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Eukaryotic translation initiation factor 3 subunit G-1 (eIF3-S4-1) CSB-YP451886DMQ
CSB-EP451886DMQ
CSB-BP451886DMQ
CSB-MP451886DMQ
CSB-EP451886DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Cytosolic Fe-S cluster assembly factor NUBP2 homolog (GK12055) CSB-YP451887DMQ
CSB-EP451887DMQ
CSB-BP451887DMQ
CSB-MP451887DMQ
CSB-EP451887DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Male-specific protein scotti (soti) CSB-YP451888DMQ
CSB-EP451888DMQ
CSB-BP451888DMQ
CSB-MP451888DMQ
CSB-EP451888DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Ribosome-releasing factor 2, mitochondrial (EF-G2), partial CSB-YP451889DMQ
CSB-EP451889DMQ
CSB-BP451889DMQ
CSB-MP451889DMQ
CSB-EP451889DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Sodium-dependent nutrient amino acid transporter 1 (NAAT1), partial CSB-YP451890DMQ
CSB-EP451890DMQ
CSB-BP451890DMQ
CSB-MP451890DMQ
CSB-EP451890DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni AT-rich binding protein (ATbp) CSB-YP451891DMQ
CSB-EP451891DMQ
CSB-BP451891DMQ
CSB-MP451891DMQ
CSB-EP451891DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Histone-arginine methyltransferase CARMER (Art4), partial CSB-YP451892DMQ
CSB-EP451892DMQ
CSB-BP451892DMQ
CSB-MP451892DMQ
CSB-EP451892DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba UPF0518 protein GE18198 (GE18198) CSB-YP451893DMR
CSB-EP451893DMR
CSB-BP451893DMR
CSB-MP451893DMR
CSB-EP451893DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Anamorsin homolog (l (2)35Bg) CSB-YP451894DMR
CSB-EP451894DMR
CSB-BP451894DMR
CSB-MP451894DMR
CSB-EP451894DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Protein Turandot M (TotM) CSB-YP451895DMR
CSB-EP451895DMR
CSB-BP451895DMR
CSB-MP451895DMR
CSB-EP451895DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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