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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila persimilis Protein wntless (wls), partial CSB-YP451836DMB1
CSB-EP451836DMB1
CSB-BP451836DMB1
CSB-MP451836DMB1
CSB-EP451836DMB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (GL13432) CSB-YP451837DMB
CSB-EP451837DMB
CSB-BP451837DMB
CSB-MP451837DMB
CSB-EP451837DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein wntless (wls), partial CSB-YP451838DMG1
CSB-EP451838DMG1
CSB-BP451838DMG1
CSB-MP451838DMG1
CSB-EP451838DMG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Male-specific protein scotti (soti) CSB-YP451839DMG
CSB-EP451839DMG
CSB-BP451839DMG
CSB-MP451839DMG
CSB-EP451839DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Transmembrane emp24 domain-containing protein eca (eca), partial CSB-YP451840DMG
CSB-EP451840DMG
CSB-BP451840DMG
CSB-MP451840DMG
CSB-EP451840DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Succinate dehydrogenase assembly factor 2-B, mitochondrial (GM20523) CSB-YP451841DMG
CSB-EP451841DMG
CSB-BP451841DMG
CSB-MP451841DMG
CSB-EP451841DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia ATPase ASNA1 homolog (GM20779) CSB-YP451842DMG
CSB-EP451842DMG
CSB-BP451842DMG
CSB-MP451842DMG
CSB-EP451842DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia CDGSH iron-sulfur domain-containing protein 2 homolog (GM12252), partial CSB-YP451843DMG
CSB-EP451843DMG
CSB-BP451843DMG
CSB-MP451843DMG
CSB-EP451843DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein phosphatase PTC7 homolog fig (fig) CSB-YP451844DMG
CSB-EP451844DMG
CSB-BP451844DMG
CSB-MP451844DMG
CSB-EP451844DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Adenosine monophosphate-protein transferase FICD homolog (GM18629), partial CSB-YP451845DMG1
CSB-EP451845DMG1
CSB-BP451845DMG1
CSB-MP451845DMG1
CSB-EP451845DMG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Eukaryotic translation initiation factor 3 subunit G-1 (eIF3-S4-1) CSB-YP451846DMG
CSB-EP451846DMG
CSB-BP451846DMG
CSB-MP451846DMG
CSB-EP451846DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein Turandot Z (TotZ) CSB-YP451847DMG
CSB-EP451847DMG
CSB-BP451847DMG
CSB-MP451847DMG
CSB-EP451847DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Eukaryotic translation initiation factor 3 subunit G-2 (eIF3-S4-2) CSB-YP451848DMG
CSB-EP451848DMG
CSB-BP451848DMG
CSB-MP451848DMG
CSB-EP451848DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Serine/threonine-protein kinase GE16371 (GE16371), partial CSB-YP451849DMR
CSB-EP451849DMR
CSB-BP451849DMR
CSB-MP451849DMR
CSB-EP451849DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Kelch-like protein diablo (dbo), partial CSB-YP451850DLL
CSB-EP451850DLL
CSB-BP451850DLL
CSB-MP451850DLL
CSB-EP451850DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Protein maelstrom (mael) CSB-YP451851DLL
CSB-EP451851DLL
CSB-BP451851DLL
CSB-MP451851DLL
CSB-EP451851DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Ribosome biogenesis protein BOP1 homolog (GH22027), partial CSB-YP451852DLL
CSB-EP451852DLL
CSB-BP451852DLL
CSB-MP451852DLL
CSB-EP451852DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Cytosolic Fe-S cluster assembly factor NUBP1 homolog (GH11560) CSB-YP451853DLL
CSB-EP451853DLL
CSB-BP451853DLL
CSB-MP451853DLL
CSB-EP451853DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta (PrBP) CSB-YP451854DLL
CSB-EP451854DLL
CSB-BP451854DLL
CSB-MP451854DLL
CSB-EP451854DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Glutamate [NMDA] receptor subunit 1 (Nmdar1), partial CSB-YP451855DLL
CSB-EP451855DLL
CSB-BP451855DLL
CSB-MP451855DLL
CSB-EP451855DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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