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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila grimshawi Serine/threonine-protein phosphatase Pgam5, mitochondrial (Pgam5) CSB-YP451856DLL
CSB-EP451856DLL
CSB-BP451856DLL
CSB-MP451856DLL
CSB-EP451856DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Elongation factor G, mitochondrial (ico), partial CSB-YP451857DLL
CSB-EP451857DLL
CSB-BP451857DLL
CSB-MP451857DLL
CSB-EP451857DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Probable ATP-dependent RNA helicase spindle-E (spn-E), partial CSB-YP451858DLL
CSB-EP451858DLL
CSB-BP451858DLL
CSB-MP451858DLL
CSB-EP451858DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Serine protease HTRA2, mitochondrial (HtrA2), partial CSB-YP451859DLL1
CSB-EP451859DLL1
CSB-BP451859DLL1
CSB-MP451859DLL1
CSB-EP451859DLL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi E3 UFM1-protein ligase 1 homolog (GH17388), partial CSB-YP451860DLL
CSB-EP451860DLL
CSB-BP451860DLL
CSB-MP451860DLL
CSB-EP451860DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Lissencephaly-1 homolog (Lis-1) CSB-YP451861DLL
CSB-EP451861DLL
CSB-BP451861DLL
CSB-MP451861DLL
CSB-EP451861DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi CDGSH iron-sulfur domain-containing protein 2 homolog (GH14305), partial CSB-YP451862DLL
CSB-EP451862DLL
CSB-BP451862DLL
CSB-MP451862DLL
CSB-EP451862DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta (PrBP) CSB-YP451863DLY
CSB-EP451863DLY
CSB-BP451863DLY
CSB-MP451863DLY
CSB-EP451863DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Elongation factor G, mitochondrial (ico), partial CSB-YP451864DLY
CSB-EP451864DLY
CSB-BP451864DLY
CSB-MP451864DLY
CSB-EP451864DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Succinate dehydrogenase assembly factor 2-A, mitochondrial (GI20197) CSB-YP451865DLY
CSB-EP451865DLY
CSB-BP451865DLY
CSB-MP451865DLY
CSB-EP451865DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Tyrosine-protein kinase-like otk (otk), partial CSB-YP451866DLY
CSB-EP451866DLY
CSB-BP451866DLY
CSB-MP451866DLY
CSB-EP451866DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Protein crossbronx-like (GI18531) CSB-YP451867DLY
CSB-EP451867DLY
CSB-BP451867DLY
CSB-MP451867DLY
CSB-EP451867DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Ubiquitin carboxyl-terminal hydrolase calypso (calypso) CSB-YP451868DLY
CSB-EP451868DLY
CSB-BP451868DLY
CSB-MP451868DLY
CSB-EP451868DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Protein alan shepard (shep), partial CSB-YP451869DLY
CSB-EP451869DLY
CSB-BP451869DLY
CSB-MP451869DLY
CSB-EP451869DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial (GI12883) CSB-YP451870DLY
CSB-EP451870DLY
CSB-BP451870DLY
CSB-MP451870DLY
CSB-EP451870DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis SAGA-associated factor 11 homolog (Sgf11) CSB-YP451871DMP
CSB-EP451871DMP
CSB-BP451871DMP
CSB-MP451871DMP
CSB-EP451871DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Kelch-like protein diablo (dbo), partial CSB-YP451872DMP
CSB-EP451872DMP
CSB-BP451872DMP
CSB-MP451872DMP
CSB-EP451872DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Ribosome biogenesis protein BOP1 homolog (GJ22246), partial CSB-YP451873DMP
CSB-EP451873DMP
CSB-BP451873DMP
CSB-MP451873DMP
CSB-EP451873DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Protein king tubby (king-tubby) CSB-YP451874DMP
CSB-EP451874DMP
CSB-BP451874DMP
CSB-MP451874DMP
CSB-EP451874DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Facilitated trehalose transporter Tret1 (Tret1), partial CSB-YP451875DMP
CSB-EP451875DMP
CSB-BP451875DMP
CSB-MP451875DMP
CSB-EP451875DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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