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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L27 (rpmA) CSB-YP452277FLX
CSB-EP452277FLX
CSB-BP452277FLX
CSB-MP452277FLX
CSB-EP452277FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP452278FLX
CSB-EP452278FLX
CSB-BP452278FLX
CSB-MP452278FLX
CSB-EP452278FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP452279FLX
CSB-EP452279FLX
CSB-BP452279FLX
CSB-MP452279FLX
CSB-EP452279FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Chromosomal replication initiator protein DnaA (dnaA) CSB-YP452280FLX
CSB-EP452280FLX
CSB-BP452280FLX
CSB-MP452280FLX
CSB-EP452280FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP452281LMV
CSB-EP452281LMV
CSB-BP452281LMV
CSB-MP452281LMV
CSB-EP452281LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Phosphopentomutase (deoB) CSB-YP452282LMV
CSB-EP452282LMV
CSB-BP452282LMV
CSB-MP452282LMV
CSB-EP452282LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP452283LMV
CSB-EP452283LMV
CSB-BP452283LMV
CSB-MP452283LMV
CSB-EP452283LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L11 (rplK) CSB-YP452284LMV
CSB-EP452284LMV
CSB-BP452284LMV
CSB-MP452284LMV
CSB-EP452284LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Recombination protein RecR (recR) CSB-YP452285LMV
CSB-EP452285LMV
CSB-BP452285LMV
CSB-MP452285LMV
CSB-EP452285LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Phosphoglycerate kinase (pgk) CSB-YP452286LMV
CSB-EP452286LMV
CSB-BP452286LMV
CSB-MP452286LMV
CSB-EP452286LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Putative Holliday junction resolvase (LAR_0521) CSB-YP452287LMV
CSB-EP452287LMV
CSB-BP452287LMV
CSB-MP452287LMV
CSB-EP452287LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP452288LMV
CSB-EP452288LMV
CSB-BP452288LMV
CSB-MP452288LMV
CSB-EP452288LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri UPF0356 protein LAR_0605 (LAR_0605) CSB-YP452289LMV
CSB-EP452289LMV
CSB-BP452289LMV
CSB-MP452289LMV
CSB-EP452289LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Trigger factor (tig) CSB-YP452290LMV
CSB-EP452290LMV
CSB-BP452290LMV
CSB-MP452290LMV
CSB-EP452290LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Ribosome maturation factor RimP (rimP) CSB-YP452291LMV
CSB-EP452291LMV
CSB-BP452291LMV
CSB-MP452291LMV
CSB-EP452291LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Chaperone protein DnaK (dnaK), partial CSB-YP452292LMV
CSB-EP452292LMV
CSB-BP452292LMV
CSB-MP452292LMV
CSB-EP452292LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri CCA-adding enzyme (cca) CSB-YP452293LMV
CSB-EP452293LMV
CSB-BP452293LMV
CSB-MP452293LMV
CSB-EP452293LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Hydroxyethylthiazole kinase (thiM) CSB-YP452294LMV
CSB-EP452294LMV
CSB-BP452294LMV
CSB-MP452294LMV
CSB-EP452294LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri tRNA (guanine-N (7)-)-methyltransferase CSB-YP452295LMV
CSB-EP452295LMV
CSB-BP452295LMV
CSB-MP452295LMV
CSB-EP452295LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP452296LMV
CSB-EP452296LMV
CSB-BP452296LMV
CSB-MP452296LMV
CSB-EP452296LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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