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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium etli 30S ribosomal protein S11 (rpsK) CSB-YP453497RKL
CSB-EP453497RKL
CSB-BP453497RKL
CSB-MP453497RKL
CSB-EP453497RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP453498RKL
CSB-EP453498RKL
CSB-BP453498RKL
CSB-MP453498RKL
CSB-EP453498RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Urease accessory protein UreE (ureE) CSB-YP453499RKL
CSB-EP453499RKL
CSB-BP453499RKL
CSB-MP453499RKL
CSB-EP453499RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Ribosome maturation factor RimP (rimP) CSB-YP453500RKL
CSB-EP453500RKL
CSB-BP453500RKL
CSB-MP453500RKL
CSB-EP453500RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Methylglyoxal synthase (mgsA) CSB-YP453501RKL
CSB-EP453501RKL
CSB-BP453501RKL
CSB-MP453501RKL
CSB-EP453501RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 5'-nucleotidase surE (surE) CSB-YP453502RKL
CSB-EP453502RKL
CSB-BP453502RKL
CSB-MP453502RKL
CSB-EP453502RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0386 protein RHECIAT_CH0001945 (RHECIAT_CH0001945) CSB-YP453503RKL
CSB-EP453503RKL
CSB-BP453503RKL
CSB-MP453503RKL
CSB-EP453503RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP453504RKL
CSB-EP453504RKL
CSB-BP453504RKL
CSB-MP453504RKL
CSB-EP453504RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Exodeoxyribonuclease 7 large subunit (xseA), partial CSB-YP453505RKL
CSB-EP453505RKL
CSB-BP453505RKL
CSB-MP453505RKL
CSB-EP453505RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP453506RKL
CSB-EP453506RKL
CSB-BP453506RKL
CSB-MP453506RKL
CSB-EP453506RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP453507RKL
CSB-EP453507RKL
CSB-BP453507RKL
CSB-MP453507RKL
CSB-EP453507RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 30S ribosomal protein S20 (rpsT) CSB-YP453508RKL
CSB-EP453508RKL
CSB-BP453508RKL
CSB-MP453508RKL
CSB-EP453508RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP453509RKL
CSB-EP453509RKL
CSB-BP453509RKL
CSB-MP453509RKL
CSB-EP453509RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP453510RKL
CSB-EP453510RKL
CSB-BP453510RKL
CSB-MP453510RKL
CSB-EP453510RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Probable GTP-binding protein EngB (engB) CSB-YP453511RKL
CSB-EP453511RKL
CSB-BP453511RKL
CSB-MP453511RKL
CSB-EP453511RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Indole-3-glycerol phosphate synthase (trpC) CSB-YP453512RKL
CSB-EP453512RKL
CSB-BP453512RKL
CSB-MP453512RKL
CSB-EP453512RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Lysine--tRNA ligase (lysS) CSB-YP453513RKL
CSB-EP453513RKL
CSB-BP453513RKL
CSB-MP453513RKL
CSB-EP453513RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Alkanesulfonate monooxygenase (ssuD) CSB-YP453514RKL
CSB-EP453514RKL
CSB-BP453514RKL
CSB-MP453514RKL
CSB-EP453514RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Integration host factor subunit beta (ihfB) CSB-YP453515RLV
CSB-EP453515RLV
CSB-BP453515RLV
CSB-MP453515RLV
CSB-EP453515RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP453516RLV
CSB-EP453516RLV
CSB-BP453516RLV
CSB-MP453516RLV
CSB-EP453516RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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